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Apparent inhibition of apoptosis by polyamines and aminothiols in DNA fragmentation assays is artifactual.
Snyder RD, Vogelpohl SJ, Baldwin BL, Deaton ED, Baden E, Carter JH. Snyder RD, et al. Cell Death Differ. 1996 Jul;3(3):323-30. Cell Death Differ. 1996. PMID: 17180101
We report that, in commonly used DNA fragmentation assays, polyamines and the radioprotective aminothiol WR1065 artifactually depress the degree of spontaneous or induced cellular apoptosis in two distinct ways. Firstly, in assays utilizing Hoechst 33258 dye to measure apo …
We report that, in commonly used DNA fragmentation assays, polyamines and the radioprotective aminothiol WR1065 artifactually depress the de …
Crohn's Disease Pathobiont Adherent-Invasive E coli Disrupts Epithelial Mitochondrial Networks With Implications for Gut Permeability.
Mancini NL, Rajeev S, Jayme TS, Wang A, Keita ÅV, Workentine ML, Hamed S, Söderholm JD, Lopes F, Shutt TE, Shearer J, McKay DM. Mancini NL, et al. Cell Mol Gastroenterol Hepatol. 2021;11(2):551-571. doi: 10.1016/j.jcmgh.2020.09.013. Epub 2020 Sep 28. Cell Mol Gastroenterol Hepatol. 2021. PMID: 32992049 Free PMC article.
Treatment with Mitochondrial Division Inhibitor 1 (Mdivi1, inhibits dynamin-related peptide 1, guanosine triphosphatase principally responsible for mitochondrial fission) or P110 (prevents dynamin-related peptide 1 binding to mitochondrial fission 1 protein) partially redu …
Treatment with Mitochondrial Division Inhibitor 1 (Mdivi1, inhibits dynamin-related peptide 1, guanosine triphosphatase principally r …