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6,756 results

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Page 1
Deuterium Solvent Kinetic Isotope Effect on Enzymatic Methyl Transfer Catalyzed by Catechol O-methyltransferase.
Zipeng Z, Fangya L, Jianyu Z. Zipeng Z, et al. Protein Pept Lett. 2023;30(4):351-359. doi: 10.2174/0929866530666230228100703. Protein Pept Lett. 2023. PMID: 36852820
RESULTS: The kinetic characteristics of COMT in H(2)O and D(2)O were significantly different under different pH/pD conditions. Significant solvent kinetic isotope effects (SKIE) were obtained, especially inverse solvent kinetic isotope effect …
RESULTS: The kinetic characteristics of COMT in H(2)O and D(2)O were significantly different under different pH/pD conditions. Significant …
Combining solvent isotope effects with substrate isotope effects in mechanistic studies of alcohol and amine oxidation by enzymes.
Fitzpatrick PF. Fitzpatrick PF. Biochim Biophys Acta. 2015 Nov;1854(11):1746-55. doi: 10.1016/j.bbapap.2014.10.020. Epub 2014 Oct 30. Biochim Biophys Acta. 2015. PMID: 25448013 Free PMC article. Review.
The inherent ambiguities in interpretation of solvent isotope effects can be significantly decreased if isotope effects arising from isotopically labeled substrates are measured in combination with solvent isotope effects …
The inherent ambiguities in interpretation of solvent isotope effects can be significantly decreased if isotope …
Inverse Solvent Isotope Effects in Enzyme-Catalyzed Reactions.
Fernandez PL, Murkin AS. Fernandez PL, et al. Molecules. 2020 Apr 21;25(8):1933. doi: 10.3390/molecules25081933. Molecules. 2020. PMID: 32326332 Free PMC article. Review.
Solvent isotope effects have long been used as a mechanistic tool for determining enzyme mechanisms. ...It is hoped that readers may find the illustrative examples valuable in planning and interpreting solvent isotope effect experiments.. …
Solvent isotope effects have long been used as a mechanistic tool for determining enzyme mechanisms. ...It is hoped tha …
Solvent isotope effect on macromolecular dynamics in E. coli.
Jasnin M, Tehei M, Moulin M, Haertlein M, Zaccai G. Jasnin M, et al. Eur Biophys J. 2008 Jun;37(5):613-7. doi: 10.1007/s00249-008-0281-4. Epub 2008 Feb 20. Eur Biophys J. 2008. PMID: 18286276
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in vivo. Measurements were performed on living E. coli bacteria containing H2O and D2O, respectively, close to physiological conditions of tem …
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in …
Solvent Deuterium Isotope Effects of Substrate Reduction by Nitrogenase from Azotobacter vinelandii.
MacArdle SG, Rees DC. MacArdle SG, et al. J Am Chem Soc. 2022 Nov 23;144(46):21125-21135. doi: 10.1021/jacs.2c07574. Epub 2022 Nov 8. J Am Chem Soc. 2022. PMID: 36346899 Free PMC article.
Nevertheless, the incorporation of H/D into products can be measured for these substrates that reflect solvent isotope effects on hydrogen atom transfers that are faster than the overall rate-determining step for nitrogenase. From these data, a minimal iso …
Nevertheless, the incorporation of H/D into products can be measured for these substrates that reflect solvent isotope effe …
Solvent dependence of the kinetic isotope effect in the reaction of ascorbate with the 2,2,6,6-tetramethylpiperidine-1-oxyl radical: tunnelling in a small molecule reaction.
Sajenko I, Pilepić V, Brala CJ, Ursić S. Sajenko I, et al. J Phys Chem A. 2010 Mar 18;114(10):3423-30. doi: 10.1021/jp911086n. J Phys Chem A. 2010. PMID: 20151699
The magnitude of the kinetic isotope effect (KIE) k(H)/k(D) in the reaction increases with decrease of the solvent polarity. ...(diox = dioxane), at 298 K; (c) the observation of isotope effect on the Arrhenius prefactor, A(H)/A(D) of 0.6(0.2) i …
The magnitude of the kinetic isotope effect (KIE) k(H)/k(D) in the reaction increases with decrease of the solvent pola …
Photosystem II oxygen-evolving complex photoassembly displays an inverse H/D solvent isotope effect under chloride-limiting conditions.
Vinyard DJ, Badshah SL, Riggio MR, Kaur D, Fanguy AR, Gunner MR. Vinyard DJ, et al. Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):18917-18922. doi: 10.1073/pnas.1910231116. Epub 2019 Sep 4. Proc Natl Acad Sci U S A. 2019. PMID: 31484762 Free PMC article.
In kinetic studies, OEC photoassembly shows a significant lag phase in H(2)O at limiting chloride concentrations with an apparent H/D solvent isotope effect of 0.14 0.05. The growth phase of OEC photoassembly shows an H/D solvent isotope effe …
In kinetic studies, OEC photoassembly shows a significant lag phase in H(2)O at limiting chloride concentrations with an apparent H/D sol …
Solvent isotope effects on alpha-glucosidase.
O'donnell AH, Yao X, Byers LD. O'donnell AH, et al. Biochim Biophys Acta. 2004 Dec 1;1703(1):63-7. doi: 10.1016/j.bbapap.2004.09.018. Biochim Biophys Acta. 2004. PMID: 15588703
The solvent kinetic isotope effects (SKIE) on the yeast alpha-glucosidase-catalyzed hydrolysis of p-nitrophenyl and methyl-d-glucopyranoside were measured at 25 degrees C. ...The initial velocities show substrate inhibition (K(is)/K(m) approximately 200) with …
The solvent kinetic isotope effects (SKIE) on the yeast alpha-glucosidase-catalyzed hydrolysis of p-nitrophenyl and met …
Solvent isotope effects in the catalytic cycle of P450 CYP17A1: Computational modeling of the hydroxylation and lyase reactions.
Denisov IG, Sligar SG. Denisov IG, et al. J Inorg Biochem. 2023 Jun;243:112202. doi: 10.1016/j.jinorgbio.2023.112202. Epub 2023 Mar 30. J Inorg Biochem. 2023. PMID: 37004494 Free PMC article.
The protonation steps are at least partially rate-limiting, therefore the steady-state rates of P450 catalysis are usually slower in deuterated solvent (D(2)O) by a factor of 1.5-3. However, in several P450 systems a pronounced inverse kinetic solvent isotope …
The protonation steps are at least partially rate-limiting, therefore the steady-state rates of P450 catalysis are usually slower in deutera …
Solvent isotope and mutagenesis studies on the proton relay system in yeast alcohol dehydrogenase 1.
Plapp BV. Plapp BV. Chem Biol Interact. 2024 Jan 25;388:110853. doi: 10.1016/j.cbi.2023.110853. Epub 2023 Dec 25. Chem Biol Interact. 2024. PMID: 38151107 Free PMC article.
The pH dependence for butanol oxidation by wild type ADH shows a wavy profile over the pH range from pH 6 to 10, with a 2.3-fold larger V(1)/K(b) in D(2)O than in H(2)O, an "inverse" isotope effect. The substrate isotope effect of 4 is not altered by t …
The pH dependence for butanol oxidation by wild type ADH shows a wavy profile over the pH range from pH 6 to 10, with a 2.3-fold larger V(1) …
6,756 results