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Joubert syndrome-derived induced pluripotent stem cells show altered neuronal differentiation in vitro.
De Mori R, Tardivo S, Pollara L, Giliani SC, Ali E, Giordano L, Leuzzi V, Fischetto R, Gener B, Diprima S, Morelli MJ, Monti MC, Sottile V, Valente EM. De Mori R, et al. Among authors: sottile v. Cell Tissue Res. 2024 Mar 19. doi: 10.1007/s00441-024-03876-9. Online ahead of print. Cell Tissue Res. 2024. PMID: 38502237
In this paper, we developed an in vitro neuronal differentiation model using patient-derived induced pluripotent stem cells (iPSCs), to evaluate possible neurodevelopmental defects in JS. To this end, iPSCs from four JS patients harboring mutations in distinct JS genes (AH …
In this paper, we developed an in vitro neuronal differentiation model using patient-derived induced pluripotent stem cells (iPSCs), …
Leptin deficiency impairs adipogenesis and browning response in mouse mesenchymal progenitors.
Velickovic K, Leija HAL, Kosic B, Sacks H, Symonds ME, Sottile V. Velickovic K, et al. Among authors: sottile v. Eur J Cell Biol. 2023 Sep;102(3):151342. doi: 10.1016/j.ejcb.2023.151342. Epub 2023 Jul 13. Eur J Cell Biol. 2023. PMID: 37467572 Free article.
The appearance of thermogenically active beige adipocytes in iWAT is known as browning. Both brown and beige cells originate from mesenchymal stem cells (MSCs), and in culture conditions a browning response can be induced with hypothermia (i.e. 32 C) during which nu …
The appearance of thermogenically active beige adipocytes in iWAT is known as browning. Both brown and beige cells originate from mes …
Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis.
Vaghi P, Oldani A, Fulghieri P, Pollara L, Valente EM, Sottile V. Vaghi P, et al. Among authors: sottile v. Methods Mol Biol. 2023;2566:53-62. doi: 10.1007/978-1-0716-2675-7_5. Methods Mol Biol. 2023. PMID: 36152242
The formation of these two lineages in cell cultures can be monitored using lipophilic dyes such as Oil Red O and substances binding to mineral deposits such as Alizarin Red S, respectively. ...Here we present the concomitant analysis of cultures containing adipogenic and …
The formation of these two lineages in cell cultures can be monitored using lipophilic dyes such as Oil Red O and substances binding …
Resveratrol and Its Analogue 4,4'-Dihydroxy-trans-stilbene Inhibit Lewis Lung Carcinoma Growth In Vivo through Apoptosis, Autophagy and Modulation of the Tumour Microenvironment in a Murine Model.
Savio M, Ferraresi A, Corpina C, Vandenberghe S, Scarlata C, Sottile V, Morini L, Garavaglia B, Isidoro C, Stivala LA. Savio M, et al. Among authors: sottile v. Biomedicines. 2022 Jul 25;10(8):1784. doi: 10.3390/biomedicines10081784. Biomedicines. 2022. PMID: 35892684 Free PMC article.
Histology was performed according to standard procedures; angiogenesis, cell proliferation and death, autophagy, infiltrating-immune cells, macrophages and fibroblasts were assessed by immunodetection assays. ...Additionally, they contrasted the tumour-permissive mi …
Histology was performed according to standard procedures; angiogenesis, cell proliferation and death, autophagy, infiltrating-immune …
In vitro Exposure to Inflammatory Mediators Affects the Differentiation of Mesenchymal Progenitors.
Marsh S, Constantin-Teodosiu T, Chapman V, Sottile V. Marsh S, et al. Among authors: sottile v. Front Bioeng Biotechnol. 2022 Jun 22;10:908507. doi: 10.3389/fbioe.2022.908507. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 35813997 Free PMC article.
However, the inflammatory environment of the joint may affect the ability of these cells to functionally integrate into the host tissue and exert beneficial effects, as hinted by a lack of success seen in clinical trials. Identification of factors and cell signallin …
However, the inflammatory environment of the joint may affect the ability of these cells to functionally integrate into the host tiss …
Patient-derived cellular models of primary ciliopathies.
Pollara L, Sottile V, Valente EM. Pollara L, et al. Among authors: sottile v. J Med Genet. 2022 Jun;59(6):517-527. doi: 10.1136/jmedgenet-2021-108315. Epub 2022 Feb 19. J Med Genet. 2022. PMID: 35184035 Review.
Primary ciliopathies are rare inherited disorders caused by structural or functional defects in the primary cilium, a subcellular organelle present on the surface of most cells. Primary ciliopathies show considerable clinical and genetic heterogeneity, with disruption of o …
Primary ciliopathies are rare inherited disorders caused by structural or functional defects in the primary cilium, a subcellular organelle …
Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
Pitrolino KA, Felfel RM, Pellizzeri LM, McLaren J, Popov AA, Sottile V, Scotchford CA, Scammell BE, Roberts GAF, Grant DM. Pitrolino KA, et al. Among authors: sottile v. Carbohydr Polym. 2022 Apr 15;282:119126. doi: 10.1016/j.carbpol.2022.119126. Epub 2022 Jan 12. Carbohydr Polym. 2022. PMID: 35123750 Free article.
The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer. ...
The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell mor …
Long-Term Culture of Stem Cells on Phosphate-Based Glass Microspheres: Synergistic Role of Chemical Formulation and 3D Architecture.
Gupta D, Hossain KMZ, Roe M, Smith EF, Ahmed I, Sottile V, Grant DM. Gupta D, et al. Among authors: sottile v. ACS Appl Bio Mater. 2021 Aug 16;4(8):5987-6004. doi: 10.1021/acsabm.1c00120. Epub 2021 Jul 29. ACS Appl Bio Mater. 2021. PMID: 35006929 Free article.
This study utilized slow-degrading (P45:45P(2)O(5)-16CaO-24MgO-11Na(2)O-4Fe(2)O(3), mol %) and comparatively faster degrading (P40:40P(2)O(5)-16CaO-24MgO-20Na(2)O, mol %) PBG microspheres with or without porosity, to evaluate the combined effect of chemical formulation and geomet …
This study utilized slow-degrading (P45:45P(2)O(5)-16CaO-24MgO-11Na(2)O-4Fe(2)O(3), mol %) and comparatively faster degrading (P40:40P(2)O(5 …
Sox-positive cell population in the adult cerebellum increases upon tissue degeneration.
Salih S, Nizamudeen ZA, De Melo N, Chakrabarti L, Sottile V. Salih S, et al. Among authors: sottile v. Exp Neurol. 2022 Feb;348:113950. doi: 10.1016/j.expneurol.2021.113950. Epub 2021 Dec 10. Exp Neurol. 2022. PMID: 34902356 Free article.
Adult neurogenesis is well-described in the subventricular and subgranular zones of the mammalian brain. Recent observations that resident glia express stem cell markers in some areas of the brain not traditionally associated with neurogenesis hint to a possible role in ti …
Adult neurogenesis is well-described in the subventricular and subgranular zones of the mammalian brain. Recent observations that resident g …
Low-Power Sonication Can Alter Extracellular Vesicle Size and Properties.
Nizamudeen ZA, Xerri R, Parmenter C, Suain K, Markus R, Chakrabarti L, Sottile V. Nizamudeen ZA, et al. Among authors: sottile v. Cells. 2021 Sep 14;10(9):2413. doi: 10.3390/cells10092413. Cells. 2021. PMID: 34572062 Free PMC article.
The present study analysed the characteristics of EVs collected from mesenchymal stem cells (MSCs) after sonication, using a combination of transmission electron microscopy, direct stochastic optical reconstruction microscopy, and flow cytometry techniques. Results showed …
The present study analysed the characteristics of EVs collected from mesenchymal stem cells (MSCs) after sonication, using a combinat …
Modulating cell differentiation in cancer models.
Fulghieri P, Stivala LA, Sottile V. Fulghieri P, et al. Among authors: sottile v. Biochem Soc Trans. 2021 Aug 27;49(4):1803-1816. doi: 10.1042/BST20210230. Biochem Soc Trans. 2021. PMID: 34436513 Review.
Cancer has been traditionally viewed as a disease characterised by excessive and uncontrolled proliferation, leading to the development of cytotoxic therapies against highly proliferating malignant cells. However, tumours frequently relapse due to the presence of slow-cycl …
Cancer has been traditionally viewed as a disease characterised by excessive and uncontrolled proliferation, leading to the development of c …
Osteogenic Response of Human Mesenchymal Stem Cells Analysed Using Combined Intracellular and Extracellular Metabolomic Monitoring.
Surrati A, Evseev S, Jourdan F, Kim DH, Sottile V. Surrati A, et al. Among authors: sottile v. Cell Physiol Biochem. 2021 Jun 19;55(3):311-326. doi: 10.33594/000000377. Cell Physiol Biochem. 2021. PMID: 34148309 Free article.
Under certain pathological conditions this remodelling balance shifts towards increased resorption resulting in weaker bone microarchitecture, and there is consequently a therapeutic need to identify pathways that could inversely enhance bone formation from stem cells. Met …
Under certain pathological conditions this remodelling balance shifts towards increased resorption resulting in weaker bone microarchitectur …
3D bioprinting of a stem cell-laden, multi-material tubular composite: An approach for spinal cord repair.
Hamid OA, Eltaher HM, Sottile V, Yang J. Hamid OA, et al. Among authors: sottile v. Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111707. doi: 10.1016/j.msec.2020.111707. Epub 2020 Nov 6. Mater Sci Eng C Mater Biol Appl. 2021. PMID: 33545866
Development of a biomimetic tubular scaffold capable of recreating developmental neurogenesis using pluripotent stem cells offers a novel strategy for the repair of spinal cord tissues. Recent advances in 3D printing technology have facilitated biofabrication of complex bi …
Development of a biomimetic tubular scaffold capable of recreating developmental neurogenesis using pluripotent stem cells offers a n …
Effect of varying the Mg with Ca content in highly porous phosphate-based glass microspheres.
Islam MT, Macri-Pellizzeri L, Hossain KMZ, Sottile V, Ahmed I. Islam MT, et al. Among authors: sottile v. Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111668. doi: 10.1016/j.msec.2020.111668. Epub 2020 Oct 22. Mater Sci Eng C Mater Biol Appl. 2021. PMID: 33545833
This paper reports on the role of phosphate-based glass (PBG) microspheres and their physicochemical properties including in vitro biological response to human mesenchymal stem cells (hMSCs). Solid and porous microspheres were prepared via a flame spheroidisation process. …
This paper reports on the role of phosphate-based glass (PBG) microspheres and their physicochemical properties including in vitro biologica …
Tailoring Pyro-and Orthophosphate Species to Enhance Stem Cell Adhesion to Phosphate Glasses.
De Melo N, Murrell L, Islam MT, Titman JJ, Macri-Pellizzeri L, Ahmed I, Sottile V. De Melo N, et al. Among authors: sottile v. Int J Mol Sci. 2021 Jan 15;22(2):837. doi: 10.3390/ijms22020837. Int J Mol Sci. 2021. PMID: 33467686 Free PMC article.
Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. ...This study highlights that tuning the composition of PBGs to possess pyro- and orthophosphate species only, enables the possibility to control cell adhesion performan …
Human mesenchymal stem cells were cultured directly onto discs made from three PBG compositions. ...This study highlights that tuning …
Rapid conversion of highly porous borate glass microspheres into hydroxyapatite.
Islam MT, Macri-Pellizzeri L, Sottile V, Ahmed I. Islam MT, et al. Among authors: sottile v. Biomater Sci. 2021 Mar 10;9(5):1826-1844. doi: 10.1039/d0bm01776k. Biomater Sci. 2021. PMID: 33459732
The in vitro SBF bioactivity studies for all the borate compositions showed HA formation and much earlier for PGMS compared to SGMS. Direct cell culture studies using hMSCs revealed that the converted porous HA microspheres showed enhanced pro-osteogenic properties compare …
The in vitro SBF bioactivity studies for all the borate compositions showed HA formation and much earlier for PGMS compared to SGMS. Direct …
In Silico Identification of SOX1 Post-Translational Modifications Highlights a Shared Protein Motif.
Ahmad A, Strohbuecker S, Scotti C, Tufarelli C, Sottile V. Ahmad A, et al. Among authors: sottile v. Cells. 2020 Nov 13;9(11):2471. doi: 10.3390/cells9112471. Cells. 2020. PMID: 33202879 Free PMC article.
The transcription factor SOX1 is a key regulator of neural stem cell development, acting to keep neural stem cells (NSCs) in an undifferentiated state. ...These results provide new research avenues for future investigations on the mechanisms regulating SOX1 activity …
The transcription factor SOX1 is a key regulator of neural stem cell development, acting to keep neural stem cells (NSCs) in a …
Targeting Glutamine Synthesis Inhibits Stem Cell Adipogenesis in Vitro.
Velickovic K, Lugo Leija HA, Surrati A, Kim DH, Sacks H, Symonds ME, Sottile V. Velickovic K, et al. Among authors: sottile v. Cell Physiol Biochem. 2020 Sep 19;54(5):917-927. doi: 10.33594/000000278. Cell Physiol Biochem. 2020. PMID: 32946687 Free article.
BACKGROUND/AIMS: Glutamine is the most abundant amino acid in the body and has a metabolic role as a precursor for protein, amino sugar and nucleotide synthesis. After glucose, glutamine is the main source of energy in cells and has recently been shown to be an important c …
BACKGROUND/AIMS: Glutamine is the most abundant amino acid in the body and has a metabolic role as a precursor for protein, amino sugar and …
Cold-induced beigeing of stem cell-derived adipocytes is not fully reversible after return to normothermia.
Lugo Leija HA, Velickovic K, Bloor I, Sacks H, Symonds ME, Sottile V. Lugo Leija HA, et al. Among authors: sottile v. J Cell Mol Med. 2020 Oct;24(19):11434-11444. doi: 10.1111/jcmm.15749. Epub 2020 Sep 9. J Cell Mol Med. 2020. PMID: 32902117 Free PMC article.
Beige adipocytes possess the morphological and biochemical characteristics of brown adipocytes, including the mitochondrial uncoupling protein (UCP)1. Mesenchymal stem cells (MSCs) are somatic multipotent progenitors which differentiate into lipid-laden adipocytes. ...Meta …
Beige adipocytes possess the morphological and biochemical characteristics of brown adipocytes, including the mitochondrial uncoupling prote …
Relating polymeric microparticle formulation to prevalence or distribution of fibronectin and poly-d-lysine to support mesenchymal stem cell growth.
Ugur D, Sottile V, Montero-Menei CN, Boury F, Zelzer M. Ugur D, et al. Among authors: sottile v. Biointerphases. 2020 Aug 4;15(4):041008. doi: 10.1116/6.0000226. Biointerphases. 2020. PMID: 32752604
Protein-coated polymer-based microparticles are attractive supports for cell delivery, but the interplay between microparticle properties, protein coating, and cell response is poorly understood. ...Furthermore, if propyleneglycol is used, the substitution of PLGA w …
Protein-coated polymer-based microparticles are attractive supports for cell delivery, but the interplay between microparticle proper …
Preclinical biological and physicochemical evaluation of two-photon engineered 3D biomimetic copolymer scaffolds for bone healing.
Kampleitner C, Changi K, Felfel RM, Scotchford CA, Sottile V, Kluger R, Hoffmann O, Grant DM, Epstein MM. Kampleitner C, et al. Among authors: sottile v. Biomater Sci. 2020 Mar 17;8(6):1683-1694. doi: 10.1039/c9bm01827a. Biomater Sci. 2020. PMID: 31984995
Two-photon stereolithography generated personalized custom-built scaffolds with a repeating 3D structure of Schwarz Primitive minimal surface unit cell with a specific pore size of 400 mum from three different methacrylated poly(d,l-lactide-co-epsilon-caprolactone) copolym …
Two-photon stereolithography generated personalized custom-built scaffolds with a repeating 3D structure of Schwarz Primitive minimal surfac …
Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture.
Popov A, Scotchford C, Grant D, Sottile V. Popov A, et al. Among authors: sottile v. Int J Mol Sci. 2019 Oct 11;20(20):5051. doi: 10.3390/ijms20205051. Int J Mol Sci. 2019. PMID: 31614651 Free PMC article.
Human mesenchymal stem cells (MSCs) show promise for musculoskeletal repair applications. ...
Human mesenchymal stem cells (MSCs) show promise for musculoskeletal repair applications. ...
Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.
De Melo N, McGinlay S, Markus R, Macri-Pellizzeri L, Symonds ME, Ahmed I, Sottile V. De Melo N, et al. Among authors: sottile v. Biomimetics (Basel). 2019 Jul 10;4(3):48. doi: 10.3390/biomimetics4030048. Biomimetics (Basel). 2019. PMID: 31295946 Free PMC article.
Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. ...Combined with the nuclear stain Hoechst 33258, this approach further enabled live confocal imaging of adipogenic ce
Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existi …
Caffeine exposure induces browning features in adipose tissue in vitro and in vivo.
Velickovic K, Wayne D, Leija HAL, Bloor I, Morris DE, Law J, Budge H, Sacks H, Symonds ME, Sottile V. Velickovic K, et al. Among authors: sottile v. Sci Rep. 2019 Jun 24;9(1):9104. doi: 10.1038/s41598-019-45540-1. Sci Rep. 2019. PMID: 31235722 Free PMC article.
This study examined the effect of caffeine on BAT thermogenesis in vitro and in vivo. Stem cell-derived adipocytes exposed to caffeine (1 mM) showed increased UCP1 protein abundance and cell metabolism with enhanced oxygen consumption and proton leak. ...
This study examined the effect of caffeine on BAT thermogenesis in vitro and in vivo. Stem cell-derived adipocytes exposed to caffein …
Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration.
Harrison R, Luckett J, Marsh S, Lugo Leija HA, Salih S, Alkharji R, Sottile V. Harrison R, et al. Among authors: sottile v. Molecules. 2019 Apr 19;24(8):1563. doi: 10.3390/molecules24081563. Molecules. 2019. PMID: 31010261 Free PMC article.
Labelled cells exhibited magnetic responsiveness, which promoted localised retention and patterned cell seeding when exposed to magnet arrangements in vitro. ...Finally, a model of cell injection into the rodent leg was used to test the enhanced localised ret …
Labelled cells exhibited magnetic responsiveness, which promoted localised retention and patterned cell seeding when exposed t …
Porous Phosphate-Based Glass Microspheres Show Biocompatibility, Tissue Infiltration, and Osteogenic Onset in an Ovine Bone Defect Model.
McLaren JS, Macri-Pellizzeri L, Hossain KMZ, Patel U, Grant DM, Scammell BE, Ahmed I, Sottile V. McLaren JS, et al. Among authors: sottile v. ACS Appl Mater Interfaces. 2019 May 1;11(17):15436-15446. doi: 10.1021/acsami.9b04603. Epub 2019 Apr 16. ACS Appl Mater Interfaces. 2019. PMID: 30990301 Free article.
With the faster degrading P40 formulation, mineralization of the tissue matrix was observed both with and without BMC. Some lymphocyte-like cells and foreign body multinucleated giant cells were observed with P40Ti2.5, suggesting that this more durable formulation m …
With the faster degrading P40 formulation, mineralization of the tissue matrix was observed both with and without BMC. Some lymphocyte-like …
Integrated Multi-Assay Culture Model for Stem Cell Chondrogenic Differentiation.
Prosser A, Scotchford C, Roberts G, Grant D, Sottile V. Prosser A, et al. Among authors: sottile v. Int J Mol Sci. 2019 Feb 22;20(4):951. doi: 10.3390/ijms20040951. Int J Mol Sci. 2019. PMID: 30813231 Free PMC article.
For this, regenerative medicine approaches require robust models to ensure selected cell populations can generate the desired cell type in a reproducible and measurable manner. ...When compared to traditional micropellets, the monolayer format appeared less able to …
For this, regenerative medicine approaches require robust models to ensure selected cell populations can generate the desired cell
Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells.
Harrison RP, Chauhan VM, Onion D, Aylott JW, Sottile V. Harrison RP, et al. Among authors: sottile v. RSC Adv. 2019 Jan 24;9(6):3176-3184. doi: 10.1039/c8ra09089k. Epub 2019 Jan 23. RSC Adv. 2019. PMID: 30774937 Free PMC article.
Silica-coated superparamagnetic iron nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell-based therapeutics and disease diagnosis. However, in order to realise their full clinical potential, their intracellular fate must be det …
Silica-coated superparamagnetic iron nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell
In vitro cellular testing of strontium/calcium substituted phosphate glass discs and microspheres shows potential for bone regeneration.
Patel U, Macri-Pellizzeri L, Zakir Hossain KM, Scammell BE, Grant DM, Scotchford CA, Hannon AC, Kennedy AR, Barney ER, Ahmed I, Sottile V. Patel U, et al. Among authors: sottile v. J Tissue Eng Regen Med. 2019 Mar;13(3):396-405. doi: 10.1002/term.2796. Epub 2019 Feb 17. J Tissue Eng Regen Med. 2019. PMID: 30666804 Free PMC article.
Cell attachment and spreading was confirmed via scanning electron microscopy (SEM) imaging at Days 3 and 14. ...Follow-on studies explored the effect of each glass composition in microsphere conformation (size: 63-125 mum) on human mesenchymal stem cells (hMSCs) in
Cell attachment and spreading was confirmed via scanning electron microscopy (SEM) imaging at Days 3 and 14. ...Follow-on studies exp
Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging.
Nizamudeen Z, Markus R, Lodge R, Parmenter C, Platt M, Chakrabarti L, Sottile V. Nizamudeen Z, et al. Among authors: sottile v. Biochim Biophys Acta Mol Cell Res. 2018 Dec;1865(12):1891-1900. doi: 10.1016/j.bbamcr.2018.09.008. Epub 2018 Oct 2. Biochim Biophys Acta Mol Cell Res. 2018. PMID: 30290236 Free PMC article.
This study introduces direct stochastic optical reconstruction microscopy (d-STORM) as an efficient and reliable characterisation approach for stem cell-derived EVs. Using a photo-switchable lipid dye, d-STORM imaging enabled rapid detection of EVs down to 20-30 nm in size …
This study introduces direct stochastic optical reconstruction microscopy (d-STORM) as an efficient and reliable characterisation approach f …
Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing.
Harrison R, Lugo Leija HA, Strohbuecker S, Crutchley J, Marsh S, Denning C, El Haj A, Sottile V. Harrison R, et al. Among authors: sottile v. Stem Cell Res Ther. 2018 Sep 26;9(1):248. doi: 10.1186/s13287-018-0968-0. Stem Cell Res Ther. 2018. PMID: 30257709 Free PMC article.
METHODS: The application of magnetic particle (MP) labelling to potentially scalable cell manufacturing processes was investigated in a range of therapeutically relevant cells, including mesenchymal stromal cells (MSC), cardiomyocytes (CMC) and neural progeni …
METHODS: The application of magnetic particle (MP) labelling to potentially scalable cell manufacturing processes was investigated in …
Flame-Spheroidized Phosphate-Based Glass Particles with Improved Characteristics for Applications in Mesenchymal Stem Cell Culture Therapy and Tissue Engineering.
Gupta D, Hossain KMZ, Ahmed I, Sottile V, Grant DM. Gupta D, et al. Among authors: sottile v. ACS Appl Mater Interfaces. 2018 Aug 8;10(31):25972-25982. doi: 10.1021/acsami.8b05267. Epub 2018 Jul 30. ACS Appl Mater Interfaces. 2018. PMID: 30011175
While most reports to date have evaluated the effect of PBG chemical formulation on bone cells, this study specifically explored the manufacturing process, the changes in physical and chemical properties of PBG particles after flame spheroidization, and subsequent effects …
While most reports to date have evaluated the effect of PBG chemical formulation on bone cells, this study specifically explored the …
Porous calcium phosphate glass microspheres for orthobiologic applications.
Hossain KMZ, Patel U, Kennedy AR, Macri-Pellizzeri L, Sottile V, Grant DM, Scammell BE, Ahmed I. Hossain KMZ, et al. Among authors: sottile v. Acta Biomater. 2018 May;72:396-406. doi: 10.1016/j.actbio.2018.03.040. Epub 2018 Mar 29. Acta Biomater. 2018. PMID: 29604438
Orthobiologics is a rapidly advancing field utilising cell-based therapies and biomaterials to enable the body to repair and regenerate musculoskeletal tissues. ...Furthermore, cytocompatibility of the microspheres was assessed using human mesenchymal stem cells via …
Orthobiologics is a rapidly advancing field utilising cell-based therapies and biomaterials to enable the body to repair and regenera …
Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro.
Velickovic K, Lugo Leija HA, Bloor I, Law J, Sacks H, Symonds M, Sottile V. Velickovic K, et al. Among authors: sottile v. Sci Rep. 2018 Mar 21;8(1):4974. doi: 10.1038/s41598-018-23267-9. Sci Rep. 2018. PMID: 29563605 Free PMC article.
We report an in vitro model to induce adipocyte browning using bone marrow (BM) derived mesenchymal stem cells (MSC), which relies on differentiation at 32 C instead of 37 C. ...Cold-exposed differentiated cells showed a marked translocation of leptin to adipocyte n …
We report an in vitro model to induce adipocyte browning using bone marrow (BM) derived mesenchymal stem cells (MSC), which relies on …
Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro.
Nizamudeen ZA, Chakrabarti L, Sottile V. Nizamudeen ZA, et al. Among authors: sottile v. Stem Cell Res. 2018 Apr;28:75-86. doi: 10.1016/j.scr.2018.02.001. Epub 2018 Feb 6. Stem Cell Res. 2018. PMID: 29448133 Free article.
While recent studies suggest that fasudil promotes the mobilization of neural stem cells (NSCs) from the subventricular zone in vivo and promotes the differentiation of the C17.2 cerebellar neuroprogenitor line in vitro, it is unclear whether fasudil is involved in the dif …
While recent studies suggest that fasudil promotes the mobilization of neural stem cells (NSCs) from the subventricular zone in vivo …
Live Quantitative Monitoring of Mineral Deposition in Stem Cells Using Tetracycline Hydrochloride.
Macri-Pellizzeri L, De Melo N, Ahmed I, Grant D, Scammell B, Sottile V. Macri-Pellizzeri L, et al. Among authors: sottile v. Tissue Eng Part C Methods. 2018 Mar;24(3):171-178. doi: 10.1089/ten.TEC.2017.0400. Epub 2018 Feb 27. Tissue Eng Part C Methods. 2018. PMID: 29353532 Free PMC article.
The final stage of in vitro osteogenic differentiation is characterized by the production of mineral deposits containing calcium cations and inorganic phosphates, which populate the extracellular matrix (ECM) surrounding the cell monolayer. Conventional histological techni …
The final stage of in vitro osteogenic differentiation is characterized by the production of mineral deposits containing calcium cations and …
Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation.
Gupta D, Grant DM, Zakir Hossain KM, Ahmed I, Sottile V. Gupta D, et al. Among authors: sottile v. J Biomater Appl. 2018 Feb;32(7):906-919. doi: 10.1177/0885328217745699. Epub 2017 Dec 13. J Biomater Appl. 2018. PMID: 29237353
In order to understand how geometry may be affecting mesenchymal stem cells, this study explored the influence of 3D geometry on mesenchymal stem cell-fate by comparing cell growth, viability and osteogenic potential using monolayer (two-dimensional, 2D) with …
In order to understand how geometry may be affecting mesenchymal stem cells, this study explored the influence of 3D geometry on mese …
Expression of a SOX1 overlapping transcript in neural differentiation and cancer models.
Ahmad A, Strohbuecker S, Tufarelli C, Sottile V. Ahmad A, et al. Among authors: sottile v. Cell Mol Life Sci. 2017 Nov;74(22):4245-4258. doi: 10.1007/s00018-017-2580-3. Epub 2017 Jul 3. Cell Mol Life Sci. 2017. PMID: 28674729 Free PMC article.
Here we report that the SOX1 locus harbours a SOX1 overlapping transcript (SOX1-OT), and describe expression, splicing variants and detection of SOX1-OT in different stem and cancer cells. RT-PCR and RACE experiments were performed to detect and characterise the structure …
Here we report that the SOX1 locus harbours a SOX1 overlapping transcript (SOX1-OT), and describe expression, splicing variants and detectio …
Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications.
Harrison R, Markides H, Morris RH, Richards P, El Haj AJ, Sottile V. Harrison R, et al. Among authors: sottile v. J Tissue Eng Regen Med. 2017 Aug;11(8):2333-2348. doi: 10.1002/term.2133. Epub 2016 May 6. J Tissue Eng Regen Med. 2017. PMID: 27151571 Free PMC article.
Following developments in isolation, expansion and differentiation protocols, efforts to promote clinical translation of emerging cellular strategies now seek to improve cell delivery and targeting. This study shows efficient live MSC labelling using silica-coated magnetic …
Following developments in isolation, expansion and differentiation protocols, efforts to promote clinical translation of emerging cellular s …
Non-destructive characterisation of mesenchymal stem cell differentiation using LC-MS-based metabolite footprinting.
Surrati A, Linforth R, Fisk ID, Sottile V, Kim DH. Surrati A, et al. Among authors: sottile v. Analyst. 2016 Jun 21;141(12):3776-87. doi: 10.1039/c6an00170j. Epub 2016 Apr 22. Analyst. 2016. PMID: 27102615
To characterise these biological changes and better understand the pathways regulating the formation of mature bone cells, the metabolic profile of mesenchymal stem cell (MSC) differentiation in vitro has been assessed non-invasively during osteogenic (OS) treatment …
To characterise these biological changes and better understand the pathways regulating the formation of mature bone cells, the metabo …
Towards quantitative molecular mapping of cells by Raman microscopy: using AFM for decoupling molecular concentration and cell topography.
Boitor R, Sinjab F, Strohbuecker S, Sottile V, Notingher I. Boitor R, et al. Among authors: sottile v. Faraday Discuss. 2016 Jun 23;187:199-212. doi: 10.1039/c5fd00172b. Faraday Discuss. 2016. PMID: 27023675
Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells in vitro. However, obtaining quantitative molecular information from Raman spectra is difficult because the intensity of a Raman band is proportional to the number of molecules in the sampled …
Raman micro-spectroscopy (RMS) is a non-invasive technique for imaging live cells in vitro. However, obtaining quantitative molecular …
Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation.
Ghita A, Pascut FC, Sottile V, Denning C, Notingher I. Ghita A, et al. Among authors: sottile v. EPJ Tech Instrum. 2015;2(1):6. doi: 10.1140/epjti/s40485-015-0016-8. Epub 2015 Mar 24. EPJ Tech Instrum. 2015. PMID: 26161299 Free PMC article.
These cells have an enormous potential for cell replacement therapies to cure diseases such as Parkinson's disease, diabetes and cardiovascular disorders, as well as in tissue engineering as a reliable cell source for providing grafts to replace and repair di …
These cells have an enormous potential for cell replacement therapies to cure diseases such as Parkinson's disease, diabetes a …
Transient accumulation of 5-carboxylcytosine indicates involvement of active demethylation in lineage specification of neural stem cells.
Wheldon LM, Abakir A, Ferjentsik Z, Dudnakova T, Strohbuecker S, Christie D, Dai N, Guan S, Foster JM, Corrêa IR Jr, Loose M, Dixon JE, Sottile V, Johnson AD, Ruzov A. Wheldon LM, et al. Among authors: sottile v. Cell Rep. 2014 Jun 12;7(5):1353-1361. doi: 10.1016/j.celrep.2014.05.003. Epub 2014 May 29. Cell Rep. 2014. PMID: 24882006 Free article.
Despite evidence that this mechanism is operative in embryonic stem cells, the role of TDG-dependent demethylation in differentiation and development is currently unclear. ...We show that 5fC and 5caC are transiently accumulated during lineage specification of neural stem …
Despite evidence that this mechanism is operative in embryonic stem cells, the role of TDG-dependent demethylation in differentiation …
Monitoring the mineralisation of bone nodules in vitro by space- and time-resolved Raman micro-spectroscopy.
Ghita A, Pascut FC, Sottile V, Notingher I. Ghita A, et al. Among authors: sottile v. Analyst. 2014 Jan 7;139(1):55-8. doi: 10.1039/c3an01716h. Epub 2013 Oct 24. Analyst. 2014. PMID: 24153382
Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells cultured in osteogenic medium in vitro. Monitoring individual bone nodules over 28 days revealed temporal and spatial changes in the crystalline ph …
Raman microscopy was used as a label-free method to study the mineralisation of bone nodules formed by mesenchymal stem cells culture …
Cytocompatibility, degradation, mechanical property retention and ion release profiles for phosphate glass fibre reinforced composite rods.
Felfel RM, Ahmed I, Parsons AJ, Palmer G, Sottile V, Rudd CD. Felfel RM, et al. Among authors: sottile v. Mater Sci Eng C Mater Biol Appl. 2013 May 1;33(4):1914-24. doi: 10.1016/j.msec.2012.12.089. Epub 2013 Jan 7. Mater Sci Eng C Mater Biol Appl. 2013. PMID: 23498213 Free article.
P40 composites exhibited good biocompatibility to human mesenchymal stem cells (MSCs), which was suggested to be due to the low degradation rate of P40 fibres. ...
P40 composites exhibited good biocompatibility to human mesenchymal stem cells (MSCs), which was suggested to be due to the low degra …
Transient serum exposure regimes to support dual differentiation of human mesenchymal stem cells.
France LA, Scotchford CA, Grant DM, Rashidi H, Popov AA, Sottile V. France LA, et al. Among authors: sottile v. J Tissue Eng Regen Med. 2014 Aug;8(8):652-63. doi: 10.1002/term.1567. Epub 2012 Nov 14. J Tissue Eng Regen Med. 2014. PMID: 23161724
Human mesenchymal stem cells (MSCs), which can generate both osteoblasts and chondrocytes, represent an ideal resource for orthopaedic repair using tissue-engineering approaches. ...
Human mesenchymal stem cells (MSCs), which can generate both osteoblasts and chondrocytes, represent an ideal resource for orthopaedi …
Cytoplasmic RNA in undifferentiated neural stem cells: a potential label-free Raman spectral marker for assessing the undifferentiated status.
Ghita A, Pascut FC, Mather M, Sottile V, Notingher I. Ghita A, et al. Among authors: sottile v. Anal Chem. 2012 Apr 3;84(7):3155-62. doi: 10.1021/ac202994e. Epub 2012 Mar 20. Anal Chem. 2012. PMID: 22436054 Free article.
Spectral images corresponding to Raman bands assigned to nucleic acids for individual NSCs and glial cells were compared with fluorescence staining of cell nuclei and cytoplasm to show that the origin of the spectral differences were related to cytoplasmic RNA. ...I …
Spectral images corresponding to Raman bands assigned to nucleic acids for individual NSCs and glial cells were compared with fluores …
5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development.
Almeida RD, Loose M, Sottile V, Matsa E, Denning C, Young L, Johnson AD, Gering M, Ruzov A. Almeida RD, et al. Among authors: sottile v. Epigenetics. 2012 Apr;7(4):383-9. doi: 10.4161/epi.19375. Epub 2012 Apr 1. Epigenetics. 2012. PMID: 22419071 Free article.
5-hydroxymethyl-cytosine (5-hmC) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (ESC) derived from mammalian blastocysts. Recent observations imply that both 5-hmC and Tet1/2/3 proteins, catalyzing the …
5-hydroxymethyl-cytosine (5-hmC) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic …
Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals.
Almeida RD, Sottile V, Loose M, De Sousa PA, Johnson AD, Ruzov A. Almeida RD, et al. Among authors: sottile v. Epigenetics. 2012 Feb;7(2):137-40. doi: 10.4161/epi.7.2.18949. Epigenetics. 2012. PMID: 22395462 Free PMC article.
Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian …
Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is …
Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin.
Rashidi H, Strohbuecker S, Jackson L, Kalra S, Blake AJ, France L, Tufarelli C, Sottile V. Rashidi H, et al. Among authors: sottile v. Cells Tissues Organs. 2012;195(6):484-94. doi: 10.1159/000329861. Epub 2011 Nov 23. Cells Tissues Organs. 2012. PMID: 22123583
Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We a …
Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro …
Epigenetic reprogramming of breast cancer cells with oocyte extracts.
Allegrucci C, Rushton MD, Dixon JE, Sottile V, Shah M, Kumari R, Watson S, Alberio R, Johnson AD. Allegrucci C, et al. Among authors: sottile v. Mol Cancer. 2011 Jan 13;10(1):7. doi: 10.1186/1476-4598-10-7. Mol Cancer. 2011. PMID: 21232089 Free PMC article.
In this study we apply the reprogramming capacity of oocytes to cancer cells in order to study breast oncogenesis. RESULTS: We show that breast cancer cells can be directly reprogrammed by amphibian oocyte extracts. ...This activity is specific to oocytes as it is n …
In this study we apply the reprogramming capacity of oocytes to cancer cells in order to study breast oncogenesis. RESULTS: We show t …
Axolotl Nanog activity in mouse embryonic stem cells demonstrates that ground state pluripotency is conserved from urodele amphibians to mammals.
Dixon JE, Allegrucci C, Redwood C, Kump K, Bian Y, Chatfield J, Chen YH, Sottile V, Voss SR, Alberio R, Johnson AD. Dixon JE, et al. Among authors: sottile v. Development. 2010 Sep;137(18):2973-80. doi: 10.1242/dev.049262. Development. 2010. PMID: 20736286 Free PMC article.
Cells in the pluripotent ground state can give rise to somatic cells and germ cells, and the acquisition of pluripotency is dependent on the expression of Nanog. ...The monomeric form is sufficient to regulate pluripotency in mouse embryonic stem cells
Cells in the pluripotent ground state can give rise to somatic cells and germ cells, and the acquisition of pluripotenc
Dynamic methylation and expression of Oct4 in early neural stem cells.
Lee SH, Jeyapalan JN, Appleby V, Mohamed Noor DA, Sottile V, Scotting PJ. Lee SH, et al. Among authors: sottile v. J Anat. 2010 Sep;217(3):203-13. doi: 10.1111/j.1469-7580.2010.01269.x. Epub 2010 Jul 14. J Anat. 2010. PMID: 20646110 Free PMC article.
Neural stem cells are a multipotent population of tissue-specific stem cells with a broad but limited differentiation potential. However, recent studies have shown that over-expression of the pluripotency gene, Oct4, alone is sufficient to initiate a process by whic …
Neural stem cells are a multipotent population of tissue-specific stem cells with a broad but limited differentiation potentia …
Variable methylation of the imprinted gene, SNRPN, supports a relationship between intracranial germ cell tumours and neural stem cells.
Lee SH, Appleby V, Jeyapalan JN, Palmer RD, Nicholson JC, Sottile V, Gao E, Coleman N, Scotting PJ. Lee SH, et al. Among authors: sottile v. J Neurooncol. 2011 Feb;101(3):419-28. doi: 10.1007/s11060-010-0275-9. Epub 2010 Jun 26. J Neurooncol. 2011. PMID: 20582452
Germ cell tumours (GCTs) are a diverse group of neoplasms all of which are generally believed to arise from germ cell progenitors (PGCs). ...Contrary to this model, we have proposed that endogenous neural stem cells of the brain are a more likely origin for t …
Germ cell tumours (GCTs) are a diverse group of neoplasms all of which are generally believed to arise from germ cell progenit …
Dynamic distribution and stem cell characteristics of Sox1-expressing cells in the cerebellar cortex.
Alcock J, Sottile V. Alcock J, et al. Among authors: sottile v. Cell Res. 2009 Dec;19(12):1324-33. doi: 10.1038/cr.2009.119. Epub 2009 Oct 13. Cell Res. 2009. PMID: 19823196
Although Bergmann glia are essential for the development and correct arborization of Purkinje cells, little is known about the regulation of this cell population after the developmental phase. ...We report that before becoming restricted to the Purkinje cell
Although Bergmann glia are essential for the development and correct arborization of Purkinje cells, little is known about the regula …
Bioluminescence imaging of human embryonic stem cells transplanted in vivo in murine and chick models.
Priddle H, Grabowska A, Morris T, Clarke PA, McKenzie AJ, Sottile V, Denning C, Young L, Watson S. Priddle H, et al. Among authors: sottile v. Cloning Stem Cells. 2009 Jun;11(2):259-67. doi: 10.1089/clo.2008.0056. Cloning Stem Cells. 2009. PMID: 19522673
Research into the behavior, efficacy, and biosafety of stem cells with a view to clinical transplantation requires the development of noninvasive methods for in vivo imaging of cells transplanted into animal models. This is particularly relevant for human embryonic …
Research into the behavior, efficacy, and biosafety of stem cells with a view to clinical transplantation requires the development of …
The chick embryo: hatching a model for contemporary biomedical research.
Rashidi H, Sottile V. Rashidi H, et al. Among authors: sottile v. Bioessays. 2009 Apr;31(4):459-65. doi: 10.1002/bies.200800168. Bioessays. 2009. PMID: 19274658 Review.
The chick embryo represents an accessible and economical in vivo model, which has long been used in developmental biology, gene expression analysis and loss/gain of function experiments. It is also an established model for tissue/cell transplantation, and because of its la …
The chick embryo represents an accessible and economical in vivo model, which has long been used in developmental biology, gene expression a …
Expression of Sox1, Sox2 and Sox9 is maintained in adult human cerebellar cortex.
Alcock J, Lowe J, England T, Bath P, Sottile V. Alcock J, et al. Among authors: sottile v. Neurosci Lett. 2009 Jan 30;450(2):114-6. doi: 10.1016/j.neulet.2008.11.047. Epub 2008 Nov 27. Neurosci Lett. 2009. PMID: 19061938
Neural stem cells (NSCs) have been found to reside in defined areas of the vertebrate brain, where they can be identified by the expression of specific markers such as Sox1, Sox2 and Sox9. In the mouse, expression of Sox1, Sox2 and Sox9 genes has recently been reported out …
Neural stem cells (NSCs) have been found to reside in defined areas of the vertebrate brain, where they can be identified by the expr …
Bone tissue formation from human embryonic stem cells in vivo.
Tremoleda JL, Forsyth NR, Khan NS, Wojtacha D, Christodoulou I, Tye BJ, Racey SN, Collishaw S, Sottile V, Thomson AJ, Simpson AH, Noble BS, McWhir J. Tremoleda JL, et al. Among authors: sottile v. Cloning Stem Cells. 2008 Mar;10(1):119-32. doi: 10.1089/clo.2007.0R36. Cloning Stem Cells. 2008. PMID: 18241129
Although the use of embryonic stem cells in the assisted repair of musculoskeletal tissues holds promise, a direct comparison of this cell source with adult marrow-derived stem cells has not been undertaken. Here we have compared the osteogenic differentiatio …
Although the use of embryonic stem cells in the assisted repair of musculoskeletal tissues holds promise, a direct comparison of this …
Human embryonic stem cells passaged using enzymatic methods retain a normal karyotype and express CD30.
Thomson A, Wojtacha D, Hewitt Z, Priddle H, Sottile V, Di Domenico A, Fletcher J, Waterfall M, Corrales NL, Ansell R, McWhir J. Thomson A, et al. Among authors: sottile v. Cloning Stem Cells. 2008 Mar;10(1):89-106. doi: 10.1089/clo.2007.0072. Cloning Stem Cells. 2008. PMID: 18241127
Human embryonic stem cells (hESCs) are thought to be susceptible to chromosomal rearrangements as a consequence of single cell dissociation. ...Cells passaged by single cell dissociation using TEG retained a normal karyotype. ...
Human embryonic stem cells (hESCs) are thought to be susceptible to chromosomal rearrangements as a consequence of single cell
Adult mesenchymal stem cells: differentiation potential and therapeutic applications.
Jackson L, Jones DR, Scotting P, Sottile V. Jackson L, et al. Among authors: sottile v. J Postgrad Med. 2007 Apr-Jun;53(2):121-7. doi: 10.4103/0022-3859.32215. J Postgrad Med. 2007. PMID: 17495381 Free article. Review.
Adult mesenchymal stem cells (MSCs) are a population of multipotent cells found primarily in the bone marrow. ...We describe here the cellular characteristics and differentiation potential of MSCs, which represent a promising stem cell population for future a …
Adult mesenchymal stem cells (MSCs) are a population of multipotent cells found primarily in the bone marrow. ...We describe h …
Bergmann glia as putative stem cells of the mature cerebellum.
Alcock J, Scotting P, Sottile V. Alcock J, et al. Among authors: sottile v. Med Hypotheses. 2007;69(2):341-5. doi: 10.1016/j.mehy.2007.01.006. Epub 2007 Mar 6. Med Hypotheses. 2007. PMID: 17337128
Recent reports now suggest the presence of such cells in the cerebellum, a part of the CNS which was not formerly thought to harbour stem cells. ...We have shown that a discrete cell population of the cerebellar cortex, the Bergmann glia, shares the expressio …
Recent reports now suggest the presence of such cells in the cerebellum, a part of the CNS which was not formerly thought to harbour …
Bone marrow as a source of stem cells and germ cells? Perspectives for transplantation.
Sottile V. Sottile V. Cell Tissue Res. 2007 Apr;328(1):1-5. doi: 10.1007/s00441-006-0361-7. Epub 2007 Jan 10. Cell Tissue Res. 2007. PMID: 17216191 Review.
Recent publications have suggested the existence of germ stem cells in the mouse at postnatal stages. The mechanism of de novo oocyte formation is proposed to involve a contribution from the bone marrow to the germ cell pool, via the bloodstream. Critical examinatio …
Recent publications have suggested the existence of germ stem cells in the mouse at postnatal stages. The mechanism of de novo oocyte …
Stem cell marker expression in the Bergmann glia population of the adult mouse brain.
Sottile V, Li M, Scotting PJ. Sottile V, et al. Brain Res. 2006 Jul 12;1099(1):8-17. doi: 10.1016/j.brainres.2006.04.127. Epub 2006 Jun 23. Brain Res. 2006. PMID: 16797497
Here we show that Sox9 is in fact also expressed in other regions harboring adult neural stem cells, suggesting that Sox9 represents a novel stem cell marker. ...Our results identify these radial glia as a previously unreported Sox1/Sox2/Sox9 positive adult cell
Here we show that Sox9 is in fact also expressed in other regions harboring adult neural stem cells, suggesting that Sox9 represents …
Multi-lineage potential of human mesenchymal stem cells following clonal expansion.
Halleux C, Sottile V, Gasser JA, Seuwen K. Halleux C, et al. Among authors: sottile v. J Musculoskelet Neuronal Interact. 2001 Sep;2(1):71-6. J Musculoskelet Neuronal Interact. 2001. PMID: 15758478 Free article.
However, previous experiments had not addressed the question whether single pluripotent stem cells exist and can give rise to these different cell lineages or whether bone marrow mesenchymal cell preparations represent a mixture of committed precursors. ...Ou …
However, previous experiments had not addressed the question whether single pluripotent stem cells exist and can give rise to these d …
Enhanced marrow adipogenesis and bone resorption in estrogen-deprived rats treated with the PPARgamma agonist BRL49653 (rosiglitazone).
Sottile V, Seuwen K, Kneissel M. Sottile V, et al. Calcif Tissue Int. 2004 Oct;75(4):329-37. doi: 10.1007/s00223-004-0224-8. Epub 2004 Jul 13. Calcif Tissue Int. 2004. PMID: 15549648
Under specific experimental conditions, these molecules induce adipogenic differentiation of mesenchymal precursor cells at the expense of osteoblasts in vitro, suggesting possible negative effects on the skeleton. ...
Under specific experimental conditions, these molecules induce adipogenic differentiation of mesenchymal precursor cells at the expen …
Immortalized fibroblast-like cells derived from human embryonic stem cells support undifferentiated cell growth.
Xu C, Jiang J, Sottile V, McWhir J, Lebkowski J, Carpenter MK. Xu C, et al. Among authors: sottile v. Stem Cells. 2004;22(6):972-80. doi: 10.1634/stemcells.22-6-972. Stem Cells. 2004. PMID: 15536188
Human embryonic stem cells (hESCs) have the potential to generate multiple cell types and hold promise for future therapeutic applications. ...These results suggest that immortalized cell lines can be generated from hESCs and that cells derived from hE …
Human embryonic stem cells (hESCs) have the potential to generate multiple cell types and hold promise for future therapeutic …
In vitro osteogenic differentiation of human ES cells.
Sottile V, Thomson A, McWhir J. Sottile V, et al. Cloning Stem Cells. 2003;5(2):149-55. doi: 10.1089/153623003322234759. Cloning Stem Cells. 2003. PMID: 12930627
Since their isolation in 1998, human embryonic stem (hES) cells have been shown to be capable of adopting various cell fates in vitro. Here, we present in vitro data demonstrating the directed commitment of human embryonic stem cells to the osteogenic lineage …
Since their isolation in 1998, human embryonic stem (hES) cells have been shown to be capable of adopting various cell fates i …
Human embryonic stem cells--realising the potential.
McWhir J, Thomson A, Sottile V. McWhir J, et al. Among authors: sottile v. Adv Exp Med Biol. 2003;534:11-25. doi: 10.1007/978-1-4615-0063-6_2. Adv Exp Med Biol. 2003. PMID: 12903708 No abstract available.
Stem cell characteristics of human trabecular bone-derived cells.
Sottile V, Halleux C, Bassilana F, Keller H, Seuwen K. Sottile V, et al. Bone. 2002 May;30(5):699-704. doi: 10.1016/s8756-3282(02)00674-9. Bone. 2002. PMID: 11996907
Human trabecular bone-derived cells (HTBs) have been used for many years as osteoblast progenitors. ...We conclude that cultures of HTBs are equivalent to cultures of "mesenchymal stem cells" (MSCs) isolated from bone marrow....
Human trabecular bone-derived cells (HTBs) have been used for many years as osteoblast progenitors. ...We conclude that cultures of H …
A high-capacity screen for adipogenic differentiation.
Sottile V, Seuwen K. Sottile V, et al. Anal Biochem. 2001 Jun 1;293(1):124-8. doi: 10.1006/abio.2001.5121. Anal Biochem. 2001. PMID: 11373088
Activity of this enzyme is therefore routinely measured to assess adipogenic differentiation in cell cultures. Existing protocols for GPDH assays require relatively large amounts of cells, and throughput is limited due to multiple steps needed for cell harves …
Activity of this enzyme is therefore routinely measured to assess adipogenic differentiation in cell cultures. Existing protocols for …
Bone morphogenetic protein-2 stimulates adipogenic differentiation of mesenchymal precursor cells in synergy with BRL 49653 (rosiglitazone).
Sottile V, Seuwen K. Sottile V, et al. FEBS Lett. 2000 Jun 23;475(3):201-4. doi: 10.1016/s0014-5793(00)01655-0. FEBS Lett. 2000. PMID: 10869556 Free article.
We confirmed the synergistic action of BMP-2 and BRL 49653 with primary cultures of rat bone marrow stromal cells. Our data demonstrate that BMP-2 can act as a potent adipogenic agent if presented together with activators of PPARgamma....
We confirmed the synergistic action of BMP-2 and BRL 49653 with primary cultures of rat bone marrow stromal cells. Our data demonstra …