Rapid and Highly Selective Fluorescent Labeling of Peptides via a Thia-Diels-Alder Cycloaddition: Application to Apelin.
Maujean T, Wagner P, Valencia C, Riché S, Iturrioz X, Villa P, Girard N, Karpenko J, Gulea M, Bonnet D.
Maujean T, et al.
Bioconjug Chem. 2023 Jan 18;34(1):162-168. doi: 10.1021/acs.bioconjchem.2c00500. Epub 2022 Dec 19.
Bioconjug Chem. 2023.
PMID: 36534753
Herein, we describe a catalyst-free thia-Diels-Alder cycloaddition for the chemoselective labeling of fully deprotected phosphonodithioester-peptides in solution with fluorophores functionalized with an exocyclic diene. The reaction was optimized on the model tripeptide …
Herein, we describe a catalyst-free thia-Diels-Alder cycloaddition for the chemoselective labeling of fully deprotected phosphonodithioester …