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Label-free, high-throughput, electrical detection of cells in droplets.
Kemna EW, Segerink LI, Wolbers F, Vermes I, van den Berg A. Kemna EW, et al. Analyst. 2013 Aug 21;138(16):4585-92. doi: 10.1039/c3an00569k. Epub 2013 Jun 10. Analyst. 2013. PMID: 23748871
Moreover, we found that the presence of a viable cell in a droplet also decreased the measured electrical impedance. This impedance change was not visible when a droplet containing a non-viable cell or an empty droplet passed the electrode pair. A
Moreover, we found that the presence of a viable cell in a droplet also decreased the measured electrical impedance. This impe …
On-chip hydrodynamic chromatography separation and detection of nanoparticles and biomolecules.
Blom MT, Chmela E, Oosterbroek RE, Tijssen R, van den Berg A. Blom MT, et al. Anal Chem. 2003 Dec 15;75(24):6761-8. doi: 10.1021/ac034663l. Anal Chem. 2003. PMID: 14670033
The hydrodynamic separations are performed in a planar configuration realized in fused silica using a mixture of fluorescent and nonfluorescent polystyrene particles with sizes ranging from 26 to 155 nm. The planar chip configuration consists of a 1-microm-hi …
The hydrodynamic separations are performed in a planar configuration realized in fused silica using a mixture of fluorescent a …
Microfabrication and microfluidics for tissue engineering: state of the art and future opportunities.
Andersson H, van den Berg A. Andersson H, et al. Lab Chip. 2004 Apr;4(2):98-103. doi: 10.1039/b314469k. Epub 2004 Mar 10. Lab Chip. 2004. PMID: 15052347 Review.
After a brief description of the background of tissue engineering, different application areas of microfluidic devices are examined. ...The first is growth of complex tissue, where microfluidic structures ensure a steady blood supply, thereby circumventing the well- …
After a brief description of the background of tissue engineering, different application areas of microfluidic devices are examined. …
Optimization of an electrolyte conductivity detector for measuring low ion concentrations.
Timmer B, Sparreboom W, Olthuis W, Bergveld P, van den Berg A. Timmer B, et al. Lab Chip. 2002 May;2(2):121-4. doi: 10.1039/b201225a. Epub 2002 Apr 17. Lab Chip. 2002. PMID: 15100845
The optimization process of a planar interdigitated conductivity detector for measuring very low electrolyte concentrations for use in a lab-on-chip gas detection system is described. ...The developed sensor has a cell constant of 7.9 m(-1) resulting in a
The optimization process of a planar interdigitated conductivity detector for measuring very low electrolyte concentrations for use i …
Microtechnologies and nanotechnologies for single-cell analysis.
Andersson H, van den Berg A. Andersson H, et al. Curr Opin Biotechnol. 2004 Feb;15(1):44-9. doi: 10.1016/j.copbio.2004.01.004. Curr Opin Biotechnol. 2004. PMID: 15102465 Review.
However, cells are nature's nanotechnology engineering at the scale of atoms and molecules, and it might be better to envision a microchip that utilizes a single cell as an experimentation platform. A novel, so-called laboratory-in-a-cell concept has b …
However, cells are nature's nanotechnology engineering at the scale of atoms and molecules, and it might be better to envision a micr …
Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection.
Vrouwe EX, Luttge R, van den Berg A. Vrouwe EX, et al. Electrophoresis. 2004 Jun;25(10-11):1660-7. doi: 10.1002/elps.200405885. Electrophoresis. 2004. PMID: 15188255
Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. ...To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis …
Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of compo …
Electrokinetic pumping and detection of low-volume flows in nanochannels.
Mela P, Tas NR, Berenschot EJ, van Nieuwkasteele J, van den Berg A. Mela P, et al. Electrophoresis. 2004 Nov;25(21-22):3687-93. doi: 10.1002/elps.200406083. Electrophoresis. 2004. PMID: 15565691
The flow was detected with the current monitoring technique by monitoring the change in resistance of the fluid in the channel upon the electroosmosis-driven displacement of an electrolyte solution by a second electrolyte solution. ...
The flow was detected with the current monitoring technique by monitoring the change in resistance of the fluid in the channel upon the elec …
Electroosmotic guiding of sample flows in a laminar flow chamber.
Besselink GA, Vulto P, Lammertink RG, Schlautmann S, van den Berg A, Olthuis W, Engbers GH, Schasfoort RB. Besselink GA, et al. Electrophoresis. 2004 Nov;25(21-22):3705-11. doi: 10.1002/elps.200406033. Electrophoresis. 2004. PMID: 15565693
The so-called address-flow principle is described: a valveless, electroosmotically driven technology used for controlling the stream profile in a laminar flow chamber. The method is explained, and a theoretical description and experimental verification are pr …
The so-called address-flow principle is described: a valveless, electroosmotically driven technology used for controlling the stream …
The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems.
Emmelkamp J, Wolbers F, Andersson H, Dacosta RS, Wilson BC, Vermes I, van den Berg A. Emmelkamp J, et al. Electrophoresis. 2004 Nov;25(21-22):3740-5. doi: 10.1002/elps.200406070. Electrophoresis. 2004. PMID: 15565697
A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. ...To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excit
A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microf
Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device.
Valero A, Merino F, Wolbers F, Luttge R, Vermes I, Andersson H, van den Berg A. Valero A, et al. Lab Chip. 2005 Jan;5(1):49-55. doi: 10.1039/b415813j. Epub 2004 Dec 6. Lab Chip. 2005. PMID: 15616740
This paper presents the design, fabrication and first results of a microfluidic cell trap device for analysis of apoptosis. The microfluidic silicon-glass chip enables the immobilization of cells and real-time monitoring of the apoptotic process. ...This paper presents the …
This paper presents the design, fabrication and first results of a microfluidic cell trap device for analysis of apoptosis. The micro …
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