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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1975 2
1976 7
1977 18
1978 44
1979 78
1980 166
1981 182
1982 264
1983 403
1984 505
1985 581
1986 686
1987 805
1988 963
1989 1128
1990 1283
1991 1278
1992 1189
1993 1112
1994 1106
1995 1157
1996 1088
1997 1065
1998 1027
1999 1063
2000 1218
2001 1256
2002 1187
2003 1543
2004 1505
2005 1572
2006 1499
2007 1500
2008 1498
2009 1413
2010 1445
2011 1442
2012 1440
2013 1257
2014 1214
2015 1087
2016 1051
2017 934
2018 859
2019 821
2020 795
2021 773
2022 660
2023 585
2024 588
2025 639
2026 437
2027 9

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45,041 results

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Page 1
On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology.
de Lorenzo V, Martínez-García E. de Lorenzo V, et al. Microb Biotechnol. 2025 Dec;18(12):e70273. doi: 10.1111/1751-7915.70273. Microb Biotechnol. 2025. PMID: 41395802 Free PMC article. Review.
Plasmid vectors are to this day the fundamental tools in molecular biology, but their selection is often guided by convenience rather than informed choice. ...The convergence of standardization and customization, enabled by advances in DNA synthesis and emerging AI- …
Plasmid vectors are to this day the fundamental tools in molecular biology, but their selection is often guided by convenience …
Nonintegrating Gene Therapy Vectors.
Athanasopoulos T, Munye MM, Yáñez-Muñoz RJ. Athanasopoulos T, et al. Hematol Oncol Clin North Am. 2017 Oct;31(5):753-770. doi: 10.1016/j.hoc.2017.06.007. Hematol Oncol Clin North Am. 2017. PMID: 28895845 Free article. Review.
However, unless engineered for replication and segregation, nonintegrating vectors will dilute progressively in proliferating cells, and are not exempt of epigenetic effects. This article provides an overview of the main nonintegrating viral (adenoviral, adeno-associated v …
However, unless engineered for replication and segregation, nonintegrating vectors will dilute progressively in proliferating cells, …
Single Plasmid-Based, Upgradable, and Backward-Compatible Adenoviral Vector Systems.
Liu H, Lu Z, Zhang X, Guo X, Mei L, Zou X, Zhong Y, Wang M, Hung T. Liu H, et al. Hum Gene Ther. 2019 Jun;30(6):777-791. doi: 10.1089/hum.2018.258. Epub 2019 Mar 27. Hum Gene Ther. 2019. PMID: 30793964
Existing adenoviral vector systems have two drawbacks. It is labor-intensive and time-consuming to load a transgene in these systems, and transgene-harboring vectors are dead ends: they cannot be reused to construct a vector carrying another transgene or achi …
Existing adenoviral vector systems have two drawbacks. It is labor-intensive and time-consuming to load a transgene in these systems, …
Cryptic resolution sites in the vector plasmid lead to the heterogeneities in the rAAV vectors.
Zhang J, Chrzanowski M, Frabutt DA, Lam AK, Mulcrone PL, Li L, Konkle BA, Miao CH, Xiao W. Zhang J, et al. J Med Virol. 2023 Jan;95(1):e28433. doi: 10.1002/jmv.28433. J Med Virol. 2023. PMID: 36571262 Free PMC article.
Conventional rAAV production primarily uses a vector plasmid as well as helper genes essential for AAV replication and packaging. Nevertheless, plasmid backbone related contaminants have been a major source of vector heterogeneity. ...By using a single …
Conventional rAAV production primarily uses a vector plasmid as well as helper genes essential for AAV replication and packagi …
Plasmid DNA and viral vector-based vaccines for the treatment of cancer.
Anderson RJ, Schneider J. Anderson RJ, et al. Vaccine. 2007 Sep 27;25 Suppl 2:B24-34. doi: 10.1016/j.vaccine.2007.05.030. Epub 2007 Jun 6. Vaccine. 2007. PMID: 17698262 Review.
Plasmid DNA and viral vector-based cancer vaccines have many inherent features that make them promising cancer vaccine candidates. This review focuses on the use of plasmid DNA and viral vector vaccines to deliver tumour-specific antigens to induce a t …
Plasmid DNA and viral vector-based cancer vaccines have many inherent features that make them promising cancer vaccine candida …
Impact of targeted vector design on Co/E1 plasmid replication.
Grabherr R, Bayer K. Grabherr R, et al. Trends Biotechnol. 2002 Jun;20(6):257-60. doi: 10.1016/s0167-7799(02)01950-9. Trends Biotechnol. 2002. PMID: 12007494 Review.
The demands for recombinant proteins, in addition to plasmid DNA, for therapeutic use are steadily increasing. Bacterial fermentation processes have long been and still are the major tool for production of these molecules. ...When high copy number plasmids are used, …
The demands for recombinant proteins, in addition to plasmid DNA, for therapeutic use are steadily increasing. Bacterial fermentation …
Enhanced design of pCMViR-TSC plasmid vector for sustainably high cargo gene expression in mammalian cells.
Sakaguchi M, Kinoshita R, Tomonobu N, Sakaguchi Y, Futami J, Yamauchi A, Murata H, Yamamoto KI, Takahashi T, Gohara Y, Ochi T, Jiang F, Komalasari NLGY, Chen Y, Ruma IMW, Sumardika IW, Zhou J, Honjo T, Kuribayashi F, Sagayama K, Toyooka S, Kondo E, Inoue Y. Sakaguchi M, et al. In Vitro Cell Dev Biol Anim. 2024 Dec;60(10):1215-1227. doi: 10.1007/s11626-024-00992-2. Epub 2024 Nov 21. In Vitro Cell Dev Biol Anim. 2024. PMID: 39570532 Free PMC article.
The first-generation pCMViR-TSC, implemented through the promoter sandwich rule, yields 10- to 100-fold higher gene expression than the standard plasmid used with the CMV (cytomegalovirus) or CAG promoter. However, the vector's shortcomings limit its utility …
The first-generation pCMViR-TSC, implemented through the promoter sandwich rule, yields 10- to 100-fold higher gene expression than the stan …
RNA-based vaccines.
Ulmer JB, Mason PW, Geall A, Mandl CW. Ulmer JB, et al. Vaccine. 2012 Jun 22;30(30):4414-8. doi: 10.1016/j.vaccine.2012.04.060. Epub 2012 Apr 28. Vaccine. 2012. PMID: 22546329 Review.
Over the past two decades, numerous clinical trials of plasmid DNA and viral vector-based vaccines have shown them to be safe, well-tolerated and immunogenic. Yet, sufficient potency for general utility in humans has remained elusive for DNA vaccines and the feasibi …
Over the past two decades, numerous clinical trials of plasmid DNA and viral vector-based vaccines have shown them to be safe, …
Single-stranded DNA plasmid, vector construction and cloning of Bacillus stearothermophilus alpha-amylase in Lactobacillus.
Cocconcelli PS, Gasson MJ, Morelli L, Bottazzi V. Cocconcelli PS, et al. Res Microbiol. 1991 Jul-Aug;142(6):643-52. doi: 10.1016/0923-2508(91)90077-n. Res Microbiol. 1991. PMID: 1961976 Free article. Review.
Vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to TaqI-digested DNA of a cryptic plasmid from Lactobacillus plantarum. ...Using plasmid pPSC22, the alpha-amylase gene of Bacillus stearothermophilus was cl …
Vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to TaqI-digested DNA of a crypt …
Multifunctional Vector for Delivery of Genome Editing Plasmid Targeting beta-Catenin to Remodulate Cancer Cell Properties.
He XY, Liu BY, Peng Y, Zhuo RX, Cheng SX. He XY, et al. ACS Appl Mater Interfaces. 2019 Jan 9;11(1):226-237. doi: 10.1021/acsami.8b17481. Epub 2018 Dec 26. ACS Appl Mater Interfaces. 2019. PMID: 30540162
Accurate and efficient delivery of genome editing plasmids to targeted cells is of critical importance in genome editing. Herein, we prepared a multifunctional delivery vector with a combination of ligand-mediated selectivity and peptide-mediated transmembrane funct …
Accurate and efficient delivery of genome editing plasmids to targeted cells is of critical importance in genome editing. Herein, we …
45,041 results
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