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Year Number of Results
1981 2
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1984 135
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1988 1357
1989 2584
1990 2951
1991 4082
1992 4364
1993 3942
1994 4148
1995 4485
1996 4923
1997 5235
1998 5371
1999 6247
2000 7307
2001 8621
2002 9102
2003 10158
2004 11345
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2007 12645
2008 13599
2009 15463
2010 18225
2011 18873
2012 18813
2013 19788
2014 19571
2015 20661
2016 18898
2017 19110
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2019 21482
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445,570 results

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Page 1
The design of a quantitative western blot experiment.
Taylor SC, Posch A. Taylor SC, et al. Biomed Res Int. 2014;2014:361590. doi: 10.1155/2014/361590. Epub 2014 Mar 16. Biomed Res Int. 2014. PMID: 24738055 Free PMC article. Review.
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a protein target in a complex sample. ...We have recently published an updated approach to produce quantitative densitometric data from wester …
Western blotting is a technique that has been in practice for more than three decades that began as a means of detecting a pro …
A systematic approach to quantitative Western blot analysis.
Pillai-Kastoori L, Schutz-Geschwender AR, Harford JA. Pillai-Kastoori L, et al. Anal Biochem. 2020 Mar 15;593:113608. doi: 10.1016/j.ab.2020.113608. Epub 2020 Jan 31. Anal Biochem. 2020. PMID: 32007473 Free article. Review.
Like with any other technology in life-sciences research, Western blotting can produce erroneous and irreproducible data. We provide a systematic approach to generate quantitative data from Western blot experiments that incorporates critical validation …
Like with any other technology in life-sciences research, Western blotting can produce erroneous and irreproducible data. We p …
In Situ Single-Cell Western Blot on Adherent Cell Culture.
Zhang Y, Naguro I, Herr AE. Zhang Y, et al. Angew Chem Int Ed Engl. 2019 Sep 23;58(39):13929-13934. doi: 10.1002/anie.201906920. Epub 2019 Aug 21. Angew Chem Int Ed Engl. 2019. PMID: 31390130 Free PMC article. Review.
Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design to eliminate the requirement for trypsin release of cells to suspension, prior to single-cell isolation and protein analysis. ...We validate …
Integrating 2D culture of adherent mammalian cells with single-cell western blotting (in situ scWB) uses microfluidic design t …
The Western Blot.
Hnasko TS, Hnasko RM. Hnasko TS, et al. Methods Mol Biol. 2015;1318:87-96. doi: 10.1007/978-1-4939-2742-5_9. Methods Mol Biol. 2015. PMID: 26160567
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. ...
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or tran …
Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans.
Springhorn A, Hoppe T. Springhorn A, et al. Methods Enzymol. 2019;619:319-336. doi: 10.1016/bs.mie.2018.12.034. Epub 2019 Feb 4. Methods Enzymol. 2019. PMID: 30910027
However, in contrast to the yeast and mammalian autophagosomal membrane proteins Atg8 and LC3, lipidation of the C. elegans ortholog LGG-1 with phosphatidylethanolamine has rarely been investigated by western blotting. We attribute this shortcoming to technical prob …
However, in contrast to the yeast and mammalian autophagosomal membrane proteins Atg8 and LC3, lipidation of the C. elegans ortholog LGG-1 w …
Protein purification and analysis: next generation Western blotting techniques.
Mishra M, Tiwari S, Gomes AV. Mishra M, et al. Expert Rev Proteomics. 2017 Nov;14(11):1037-1053. doi: 10.1080/14789450.2017.1388167. Epub 2017 Oct 13. Expert Rev Proteomics. 2017. PMID: 28974114 Free PMC article. Review.
Western blotting is one of the most commonly used techniques in molecular biology and proteomics. ...Expert commentary: Over the last decade significant improvements have been made in creating more sensitive, automated, and advanced techniques by optimizing various …
Western blotting is one of the most commonly used techniques in molecular biology and proteomics. ...Expert commentary: Over t …
Western blotting: a powerful staple in scientific and biomedical research.
Begum H, Murugesan P, Tangutur AD. Begum H, et al. Biotechniques. 2022 Jun;73(1):58-69. doi: 10.2144/btn-2022-0003. Epub 2022 Jul 1. Biotechniques. 2022. PMID: 35775367 Free article. Review.
Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the protein, ranging from basic protein analysis to disease detection. ...
Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to inv …
Extracellular Vesicle Isolation and Analysis by Western Blotting.
Kowal EJK, Ter-Ovanesyan D, Regev A, Church GM. Kowal EJK, et al. Methods Mol Biol. 2017;1660:143-152. doi: 10.1007/978-1-4939-7253-1_12. Methods Mol Biol. 2017. PMID: 28828654
Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9 , CD63 and CD81 ) by western blotting....
Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspani …
Western Blot Processing Optimization: The Perfect Blot.
Yukhananov R, Chimento DP, Marlow LA. Yukhananov R, et al. Methods Mol Biol. 2022;2349:65-80. doi: 10.1007/978-1-0716-1585-0_4. Methods Mol Biol. 2022. PMID: 34718991
Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electrophoresis separation, transfer to a membrane, and immunodetection with specific antibodies. ...
Western blot processing is a well-established procedure that includes protein extraction from tissues and cells, gel electroph …
Core-shell SERS nanotags-based western blot.
Liu B, Tang H, Liu Q, Wang W, Li H, Zheng S, Sun F, Zhao X. Liu B, et al. Talanta. 2023 Feb 1;253:123888. doi: 10.1016/j.talanta.2022.123888. Epub 2022 Sep 3. Talanta. 2023. PMID: 36087412
Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based …
Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challe …
445,570 results
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