Arabidopsis UEV1D promotes Lysine-63-linked polyubiquitination and is involved in DNA damage response

Plant Cell. 2008 Jan;20(1):213-27. doi: 10.1105/tpc.107.051862. Epub 2008 Jan 4.

Abstract

DNA damage tolerance (DDT) in budding yeast requires Lys-63-linked polyubiquitination of the proliferating cell nuclear antigen. The ubiquitin-conjugating enzyme Ubc13 and the Ubc enzyme variant (Uev) methyl methanesulfonate2 (Mms2) are required for this process. Mms2 homologs have been found in all eukaryotic genomes examined; however, their roles in multicellular eukaryotes have not been elucidated. We report the isolation and characterization of four UEV1 genes from Arabidopsis thaliana. All four Uev1 proteins can form a stable complex with At Ubc13 or with Ubc13 from yeast or human and can promote Ubc13-mediated Lys-63 polyubiquitination. All four Uev1 proteins can replace yeast MMS2 DDT functions in vivo. Although these genes are ubiquitously expressed in most tissues, UEV1D appears to express at a much higher level in germinating seeds and in pollen. We obtained and characterized two uev1d null mutant T-DNA insertion lines. Compared with wild-type plants, seeds from uev1d null plants germinated poorly when treated with a DNA-damaging agent. Those that germinated grew slower, and the majority ceased growth within 2 weeks. Pollen from uev1d plants also displayed a moderate but significant decrease in germination in the presence of DNA damage. This report links Ubc13-Uev with functions in DNA damage response in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • DNA Damage*
  • DNA, Bacterial
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant
  • Genetic Complementation Test
  • Germination / drug effects
  • Lysine / metabolism*
  • Methyl Methanesulfonate / pharmacology
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutation / genetics
  • Phenotype
  • Pollen / drug effects
  • Pollen / metabolism
  • Polyubiquitin / metabolism*
  • Protein Binding / drug effects
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / metabolism
  • Seeds / drug effects
  • Seeds / metabolism
  • Ubiquitin-Conjugating Enzymes / chemistry
  • Ubiquitin-Conjugating Enzymes / genetics
  • Ubiquitin-Conjugating Enzymes / metabolism*
  • Ubiquitin-Protein Ligases
  • Ubiquitination* / drug effects

Substances

  • Arabidopsis Proteins
  • DNA, Bacterial
  • MMS2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • T-DNA
  • UEV1D protein, Arabidopsis
  • Polyubiquitin
  • Methyl Methanesulfonate
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligases
  • Lysine

Associated data

  • RefSeq/NP_003341
  • RefSeq/NP_011428
  • RefSeq/NP_076074
  • RefSeq/NP_493578
  • RefSeq/NP_564994
  • RefSeq/NP_565834
  • RefSeq/NP_566968
  • RefSeq/NP_588162
  • RefSeq/NP_647959
  • RefSeq/NP_850259