This study attempted to reconstruct deformities of a Poland syndrome patient using autologous fat tissues. All injected fat tissues were condensed by squeezing centrifugation. Operations were performed four times with intervals over 6 months. The total injection volume was 972 ml, and the maintained volume of 628 ml was measured by means of a magnetic resonance image (MRI). The entire follow-up period was 4.5 years. After surgery, several small cysts and minimal calcifications were present but no significant complications. The cosmetic outcomes and volume maintenance rates were excellent despite the overlapped large-volume injections. In conclusion, higher condensation of fat tissues through squeezing centrifugation would help to achieve better results in volume maintenance and reduce complications. It is necessary, however, to perform more comparative studies with many clinical cases for a more scientific analysis. The study experiments with squeezed fat simply suggest a hypothesis that squeezing centrifugation could select healthier cells through pressure disruption of relatively thinner membranes of larger, more vulnerable and more mature fat cells.