Plasmid mapping computer program

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):717-29. doi: 10.1093/nar/12.1part2.717.

Abstract

Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence*
  • Computers*
  • DNA / genetics
  • DNA Restriction Enzymes
  • Methods
  • Microcomputers*
  • Plasmids*
  • Software*

Substances

  • DNA
  • DNA Restriction Enzymes