Magnetic Particle Imaging tracks the long-term fate of in vivo neural cell implants with high image contrast

Sci Rep. 2015 Sep 11:5:14055. doi: 10.1038/srep14055.

Abstract

We demonstrate that Magnetic Particle Imaging (MPI) enables monitoring of cellular grafts with high contrast, sensitivity, and quantitativeness. MPI directly detects the intense magnetization of iron-oxide tracers using low-frequency magnetic fields. MPI is safe, noninvasive and offers superb sensitivity, with great promise for clinical translation and quantitative single-cell tracking. Here we report the first MPI cell tracking study, showing 200-cell detection in vitro and in vivo monitoring of human neural graft clearance over 87 days in rat brain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Tracking* / methods
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / metabolism
  • Female
  • Humans
  • Iron / metabolism
  • Magnetic Resonance Imaging* / methods
  • Magnetite Nanoparticles*
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Staining and Labeling

Substances

  • Magnetite Nanoparticles
  • Iron