Fabrication of universal serial bus flash disk type microfluidic chip electrophoresis and application for protein analysis under ultra low voltage

Hailin Cong; Xiaodan Xu; Bing Yu; Huwei Liu; Hua Yuan

doi:10.1063/1.4943915

Fabrication of universal serial bus flash disk type microfluidic chip electrophoresis and application for protein analysis under ultra low voltage

Biomicrofluidics. 2016 Mar 15;10(2):024107. doi: 10.1063/1.4943915. eCollection 2016 Mar.

Authors

Hailin Cong, Xiaodan Xu¹, Bing Yu, Huwei Liu², Hua Yuan¹

Affiliations

¹ College of Materials Science and Engineering, Qingdao University , Qingdao 266071, China.
² Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.

Abstract

A simple and effective universal serial bus (USB) flash disk type microfluidic chip electrophoresis (MCE) was developed by using poly(dimethylsiloxane) based soft lithography and dry film based printed circuit board etching techniques in this paper. The MCE had a microchannel diameter of 375 μm and an effective length of 25 mm. Equipped with a conventional online electrochemical detector, the device enabled effectively separation of bovine serum albumin, lysozyme, and cytochrome c in 80 s under the ultra low voltage from a computer USB interface. Compared with traditional capillary electrophoresis, the USB flash disk type MCE is not only portable and inexpensive but also fast with high separation efficiency.