Genome-wide association meta-analyses to identify common genetic variants associated with hallux valgus in Caucasian and African Americans

J Med Genet. 2015 Nov;52(11):762-9. doi: 10.1136/jmedgenet-2015-103142. Epub 2015 Sep 2.

Abstract

Objective: Hallux valgus (HV) affects ∼36% of Caucasian adults. Although considered highly heritable, the underlying genetic determinants are unclear. We conducted the first genome-wide association study (GWAS) aimed to identify genetic variants associated with HV.

Methods: HV was assessed in three Caucasian cohorts (n=2263, n=915 and n=1231 participants, respectively). In each cohort, a GWAS was conducted using 2.5 M imputed SNPs. Mixed-effect regression with the additive genetic model adjusted for age, sex, weight and within-family correlations was used for both sex-specific and combined analyses. To combine GWAS results across cohorts, fixed-effect inverse-variance meta-analyses were used. Following meta-analyses, top-associated findings were also examined in an African American cohort (n=327).

Results: The proportion of HV variance explained by genome-wide genotyped SNPs was 50% in men and 48% in women. A higher proportion of genetic determinants of HV were sex specific. The most significantly associated SNP in men was rs9675316 located on chr17q23-a24 near the AXIN2 gene (p=0.000000546×10(-7)); the most significantly associated SNP in women was rs7996797 located on chr13q14.1-q14.2 near the ESD gene (p=0.000000721×10(-7)). Genome-wide significant SNP-by-sex interaction was found for SNP rs1563374 located on chr11p15.1 near the MRGPRX3 gene (interaction p value =0.0000000041×10(-9)). The association signals diminished when combining men and women.

Conclusions: The findings suggest that the potential pathophysiological mechanisms of HV are complex and strongly underlined by sex-specific interactions. The identified genetic variants imply contribution of biological pathways observed in osteoarthritis as well as new pathways, influencing skeletal development and inflammation.

Keywords: GWAS; candidate genes; genetic variants; hallux valgus.

Publication types

  • Meta-Analysis
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Axin Protein / genetics
  • Black or African American / genetics*
  • Carboxylesterase / genetics
  • Female
  • Genetic Predisposition to Disease
  • Genome-Wide Association Study
  • Hallux Valgus / genetics*
  • Humans
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Receptors, G-Protein-Coupled / genetics
  • Sex Factors
  • White People / genetics*

Substances

  • AXIN2 protein, human
  • Axin Protein
  • MRGPRX3 protein, human
  • Receptors, G-Protein-Coupled
  • Carboxylesterase
  • ESD protein, human