Discriminant Analysis of Defective and Non-Defective Field Pea (Pisum sativum L.) into Broad Market Grades Based on Digital Image Features

PLoS One. 2016 May 13;11(5):e0155523. doi: 10.1371/journal.pone.0155523. eCollection 2016.

Abstract

Field peas (Pisum sativum L.) are generally traded based on seed appearance, which subjectively defines broad market-grades. In this study, we developed an objective Linear Discriminant Analysis (LDA) model to classify market grades of field peas based on seed colour, shape and size traits extracted from digital images. Seeds were imaged in a high-throughput system consisting of a camera and laser positioned over a conveyor belt. Six colour intensity digital images were captured (under 405, 470, 530, 590, 660 and 850nm light) for each seed, and surface height was measured at each pixel by laser. Colour, shape and size traits were compiled across all seed in each sample to determine the median trait values. Defective and non-defective seed samples were used to calibrate and validate the model. Colour components were sufficient to correctly classify all non-defective seed samples into correct market grades. Defective samples required a combination of colour, shape and size traits to achieve 87% and 77% accuracy in market grade classification of calibration and validation sample-sets respectively. Following these results, we used the same colour, shape and size traits to develop an LDA model which correctly classified over 97% of all validation samples as defective or non-defective.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calibration
  • Discriminant Analysis*
  • Image Processing, Computer-Assisted*
  • Marketing*
  • Models, Biological
  • Pisum sativum / anatomy & histology*
  • Pisum sativum / physiology
  • Reproducibility of Results
  • Seeds / physiology

Associated data

  • figshare/10.6084/m9.figshare.3273898

Grants and funding

This project was supported by the Grains Research and Development Corporation through grant DAV00132 and the Department of Economic Development, Jobs, Transport and Resources. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.