Abstract
Hypoxia-inducible factor (HIF)-1alpha and -2alpha are two basic helix-loop-helix/PAS domain transcriptional factors that mediate hypoxia-induced gene expression. We found that bovine arterial endothelial cells (BAEC) expressed both HIF-1alpha and -2alpha by RT-PCR and then isolated cDNAs encoding these two transcriptional factors of BAEC. The deduced amino acid sequences of both HIF-1alpha and -2alpha showed high homologies among mammalian species. Northern blot analysis indicated that the mRNAs for HIF-1alpha and -2alpha from BAEC showed a size of approx. 5.5 and 6.2 kilobases, respectively and that both mRNAs were constitutively expressed and not induced by hypoxia in BAEC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Basic Helix-Loop-Helix Transcription Factors
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Cattle
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary / chemistry*
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DNA, Complementary / isolation & purification
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DNA-Binding Proteins*
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Endothelium, Vascular / metabolism*
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Gene Expression Regulation
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Molecular Sequence Data
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Nuclear Proteins*
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Sequence Alignment
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Trans-Activators / genetics*
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Trans-Activators / metabolism*
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Transcription Factors*
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA, Complementary
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DNA-Binding Proteins
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Nuclear Proteins
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Trans-Activators
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Transcription Factors
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endothelial PAS domain-containing protein 1
Associated data
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GENBANK/AB018398
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GENBANK/AB018399