Abstract
The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCFTIR1. The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCFTIR1 is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arabidopsis / drug effects
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Arabidopsis / enzymology*
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis Proteins*
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Gene Expression Regulation, Plant
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Indoleacetic Acids / pharmacology
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Molecular Sequence Data
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Peptide Synthases / genetics
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Peptide Synthases / physiology*
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Plant Proteins / genetics
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Plant Proteins / physiology*
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Plant Roots / growth & development
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Recombinant Fusion Proteins / physiology
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SKP Cullin F-Box Protein Ligases
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Sequence Alignment
Substances
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ASK1 protein, Arabidopsis
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Arabidopsis Proteins
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Indoleacetic Acids
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Plant Proteins
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Recombinant Fusion Proteins
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indoleacetic acid
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ASK2 protein, Arabidopsis
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SKP Cullin F-Box Protein Ligases
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Peptide Synthases