Selective coupling of mouse brain metabotropic sigma receptor with recombinant Gi1

Neurosci Lett. 1999 Jun 18;268(2):85-8. doi: 10.1016/s0304-3940(99)00387-0.

Abstract

Various sigma (sigma) ligands including (+)-pentazocine stimulated [35S]GTPgammaS binding in synaptic membranes from the mouse cerebellum. The (+)-pentazocine-stimulated [35S]GTPgammaS binding was blocked by the treatment of membranes with pertussis toxin (PTX), but completely recovered by the reconstitution of PTX-treated membranes with recombinant Gi1, but not with GoA. These findings suggest that metabotropic sigma receptors are selectively coupled to Gi1 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / pharmacology
  • Brain Chemistry*
  • Dimerization
  • GTP-Binding Protein alpha Subunits, Gi-Go / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
  • Macromolecular Substances
  • Male
  • Mice
  • Mice, Mutant Strains
  • Pentazocine / pharmacology
  • Pertussis Toxin
  • Receptors, sigma / metabolism*
  • Recombinant Proteins / metabolism*
  • Sulfur Radioisotopes / metabolism
  • Synaptic Membranes / drug effects
  • Synaptic Membranes / metabolism
  • Virulence Factors, Bordetella / pharmacology

Substances

  • Bacterial Proteins
  • Macromolecular Substances
  • Receptors, sigma
  • Recombinant Proteins
  • Sulfur Radioisotopes
  • Virulence Factors, Bordetella
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Pertussis Toxin
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Pentazocine