Abstract
We have studied two anti-p185 antibodies: the monoclonal antibody 7. 16.4 and rhuMAb 4D5, which were raised against the the ectodomain of rat (p185(neu)), and the human (p185(her2/neu)) homolog, respectively. Studies on the structure of these two antibodies indicate that they share structural similarity in the variable region, especially the CDR3 region, which determines the antibody-antigen interaction. Further studies by flow cytometry revealed that 7.16.4 can compete with rhuMAb4D5 for binding to the cell surface p185(her2/neu), suggesting that these two antibodies share an epitope on the p185 receptor. Furthermore, 7.16.4 can also inhibit proliferation and transformation caused by p185(her2/neu). Moreover the rhuMAb 4D5 binds to the rat p185(neu). With the observation that 7.16.4 positively stains human breast cancer tissues that overexpress p185(her2/neu), 7.16.4 may be useful for the pathological diagnosis and therapy of human tumors.
Copyright 1999 Academic Press.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / physiology*
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Antibodies, Monoclonal, Humanized
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Binding Sites, Antibody
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Cell Transformation, Neoplastic
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Cross Reactions / immunology
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Epitopes / immunology*
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Female
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Flow Cytometry
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Humans
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Immunoenzyme Techniques
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Immunoglobulin Variable Region / chemistry
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Immunoglobulin Variable Region / immunology
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Male
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Mice
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Models, Molecular
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / pathology
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Rats
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Receptor, ErbB-2 / immunology*
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Receptor, ErbB-2 / metabolism
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Tetrazolium Salts / metabolism
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Thiazoles / metabolism
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Trastuzumab
Substances
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Antibodies, Monoclonal
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Antibodies, Monoclonal, Humanized
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Epitopes
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Immunoglobulin Variable Region
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Tetrazolium Salts
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Thiazoles
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Receptor, ErbB-2
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thiazolyl blue
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Trastuzumab