Nine cases of single segment vertebral osteomyelitis were included based on the single level of vertebral body involvement according to the MR findings. They were 3 cases with tuberculous infection and 6 cases with pyogenic infection. The vertebral body involvement was presented as abnormal signal changes (100%) and heterogenous enhancement (77.7%). They usually caused the cortical disruption in its anterior aspect (100%). It goes along the upward subligamentous spread (100%) most often, then the upper disc involvement (66.6%) and downward subligamentous spread (55.5%). The lower disc involvement is least common (11.1%). By using these criteria, the single segment vertebral osteomyelitis could be earlier diagnosed.