Characterization of an alpha-tubulin gene of Cryptosporidium parvum

M T Bonafonte; D Garmon; J R Mead

doi:10.1111/j.1550-7408.1999.tb06073.x

Characterization of an alpha-tubulin gene of Cryptosporidium parvum

J Eukaryot Microbiol. 1999 Sep-Oct;46(5):545-7. doi: 10.1111/j.1550-7408.1999.tb06073.x.

Authors

M T Bonafonte¹, D Garmon, J R Mead

Affiliation

¹ VA Medical Center, Decatur, Georgia 30033, USA.

PMID: 10519222
DOI: 10.1111/j.1550-7408.1999.tb06073.x

Abstract

A gene encoding an alpha-tubulin of Cryptosporidium parvum was isolated and characterized. It had no introns, and encoded a 441-amino acid protein whose predicted ORF represented a typical alpha-tubulin protein with a MW of 50.5 kDa. This tubulin had an amino acid sequence similarity with Apicomplexa Plasmodium falciparum and Toxoplasma gondii higher than 88% and shared a number of conserved motifs.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Blotting, Western
Cryptosporidium parvum / genetics*
DNA, Protozoan / genetics
Genes, Protozoan*
Molecular Sequence Data
Polymerase Chain Reaction / methods
RNA, Protozoan / genetics
Reverse Transcriptase Polymerase Chain Reaction / methods
Sequence Analysis, DNA
Tubulin / genetics*
Tubulin / metabolism*

Substances

DNA, Protozoan
RNA, Protozoan
Tubulin

Associated data

GENBANK/AF082877