Yeast ascospore wall assembly requires two chitin deacetylase isozymes

FEBS Lett. 1999 Oct 29;460(2):275-9. doi: 10.1016/s0014-5793(99)01334-4.

Abstract

Chitin deacetylases are required for spore wall rigidity in Saccharomyces cerevisiae. Two chitin deacetylase genes (CDA1 and CDA2) have been identified in yeast. In this report we studied the biochemical properties of the chitin deacetylases encoded by CDA1 and CDA2 and we show how their elimination directly affects the ascospore wall assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / pharmacology
  • Amidohydrolases / genetics
  • Amidohydrolases / physiology*
  • Cell Division / genetics
  • Cell Wall / enzymology*
  • Cell Wall / physiology
  • Gene Expression Regulation, Fungal
  • Glucosamine / metabolism
  • Isoenzymes
  • Mutagenesis
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Time Factors
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism

Substances

  • Isoenzymes
  • Tyrosine
  • dityrosine
  • Amidohydrolases
  • chitin deacetylase
  • Glucosamine
  • Acetic Acid