Abstract
The channel-associated protein PSD-95 functionally modulates NMDA receptor channels, interacting with the channels via PDZ domain of PSD-95. PSD-95 also interacts with guanylate kinase-associated protein (GKAP) through the guanylate kinase-like domain of PSD-95. Here we report that GKAP markedly potentiates the channel activity of the receptor-PSD-95 complex. However, GKAP had no effect on basic properties of the channels nor on PSD-95-induced changes in channel properties. Thus, GKAP affects the channel activity of the NMDA receptor via PSD-95 quantitatively, which may make signal transmission more efficient at postsynaptic sites.
MeSH terms
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Animals
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Carrier Proteins / pharmacology*
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Gene Expression
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Glutamic Acid / pharmacology
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Ion Channels / genetics
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Microinjections
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Mutation
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Oocytes
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Patch-Clamp Techniques
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RNA, Messenger / genetics
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Receptors, N-Methyl-D-Aspartate / genetics
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Receptors, N-Methyl-D-Aspartate / metabolism*
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SAP90-PSD95 Associated Proteins
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Tetradecanoylphorbol Acetate / pharmacology
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Xenopus laevis
Substances
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Carrier Proteins
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Ion Channels
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Nerve Tissue Proteins
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RNA, Messenger
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Receptors, N-Methyl-D-Aspartate
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SAP90-PSD95 Associated Proteins
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postsynaptic density proteins
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Glutamic Acid
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Tetradecanoylphorbol Acetate