High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome

M G Thomas; N Bradman; H M Flinn

doi:10.1007/s004399900181

High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome

Hum Genet. 1999 Dec;105(6):577-81. doi: 10.1007/s004399900181.

Authors

M G Thomas¹, N Bradman, H M Flinn

Affiliation

¹ The Centre for Genetic Anthropology, The Department of Anthropology, University College London, UK. m.thomas@ucl.ac.uk

PMID: 10647892
DOI: 10.1007/s004399900181

Abstract

We describe an integrated approach to the determination of complex Y chromosome haplotypes that is both fast and relatively inexpensive. The method employs GeneScan technology to enable a researcher to assay repeat number variation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs per sample and is relatively insensitive to sample DNA concentration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genotype
Haplotypes
Humans
Male
Microsatellite Repeats
Polymerase Chain Reaction
Polymorphism, Genetic
Reagent Kits, Diagnostic
Sensitivity and Specificity
Y Chromosome / genetics*

Substances

Reagent Kits, Diagnostic