Abstract
We assessed the effect of rIL-12 on the expression of class II molecules and on the ratio between SDS-stable and unstable alphabeta dimers in dendritic cells. We found that in vitro exposure of the cells to IL-12 increased their surface expression of mature class II molecules, despite a marked decline in class II biosynthesis. This effect was accompanied by a striking increase in the overall proportion of SDS-stable heterodimers.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western / methods
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Dimerization
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Histocompatibility Antigens Class II / biosynthesis*
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Interleukin-12 / immunology*
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Interleukin-12 / pharmacology
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Mice
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Mice, Inbred DBA
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Sodium Dodecyl Sulfate*
Substances
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Histocompatibility Antigens Class II
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I-Ad antigen
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Interleukin-12
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Sodium Dodecyl Sulfate