Abstract
Membrane depolarization of neurons is thought to lead to changes in gene expression that modulate neuronal plasticity. We used representational difference analysis to identify a group of cDNAs that are induced by membrane depolarization or by forskolin, but not by neurotrophins or growth factors, in PC12 pheochromocytoma cells. One of these genes, SIK (salt-inducible kinase), is a member of the sucrose-nonfermenting 1 protein kinase/AMP-activated protein kinase protein kinase family that was also recently identified from the adrenal gland of rats treated with high-salt diets. SIK mRNA is induced up to eightfold in specific regions of the hippocampus and cortex in rats, following systemic kainic acid administration and seizure induction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Blotting, Northern
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Cerebral Cortex / cytology
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Cerebral Cortex / enzymology*
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Colforsin / pharmacology
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Cycloheximide / pharmacology
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Epilepsy / chemically induced
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Epilepsy / physiopathology
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Excitatory Amino Acid Agonists
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Gene Expression Regulation, Enzymologic / physiology*
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Genes, Immediate-Early / drug effects
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Genes, Immediate-Early / physiology
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Hippocampus / cytology
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Hippocampus / enzymology
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Kainic Acid
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Molecular Sequence Data
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Neurons / enzymology*
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PC12 Cells
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Phylogeny
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Protein Synthesis Inhibitors / pharmacology
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Protein-Tyrosine Kinases
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RNA, Messenger / metabolism
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Rats
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Salts / metabolism
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Seizures / chemically induced
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Seizures / physiopathology
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Sequence Homology, Amino Acid
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Serine / metabolism
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src-Family Kinases / genetics*
Substances
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Excitatory Amino Acid Agonists
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Protein Synthesis Inhibitors
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RNA, Messenger
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Salts
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Colforsin
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Serine
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Cycloheximide
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Protein-Tyrosine Kinases
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Ptk6 protein, mouse
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src-Family Kinases
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Kainic Acid