Genetic analysis of the ycgJ-metB-cysK-ygaG operon negatively regulated by the VirR/VirS system in Clostridium perfringens

Microbiol Immunol. 2000;44(6):525-8. doi: 10.1111/j.1348-0421.2000.tb02528.x.

Abstract

The 5'-flanking region of the metB-cysK-ygaG operon, whose expression is negatively regulated by the VirR/VirS system in C. perfringens, was analyzed. The region contained the ycgJ, mscL, and colA genes encoding a hypothetical protein, a large conductance mechanosensitive channel protein, and kappa-toxin (collagenase), respectively. Northern analysis revealed that the ycgJ gene was transcribed as a 4.9-kb operon together with the metB-cysK-ygaG genes and that this operon was negatively regulated by the VirR/VirS system. It is indicated that the pfoA (theta-toxin or perfringolysin O), colA, and ycgJ-metB-cysK-ygaG genes that belong to the VirR/VirS regulon are situated very close together in a 26.5-kb region of the chromosome, but do not form a pathogenic island.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Carbon-Oxygen Lyases / genetics*
  • Clostridium perfringens / genetics*
  • Cysteine Synthase / genetics*
  • Gene Expression Regulation, Bacterial*
  • Gene Expression Regulation, Enzymologic*
  • Operon*
  • RNA, Bacterial
  • RNA, Messenger

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • RNA, Messenger
  • mry protein, Streptococcus pyogenes
  • Cysteine Synthase
  • O-succinylhomoserine (thiol)-lyase
  • Carbon-Oxygen Lyases