Analysis of genetic heterogeneity in Chlamydia trachomatis clinical isolates of serovars D, E, and F by amplified fragment length polymorphism

S A Morré; J M Ossewaarde; P H Savelkoul; J Stoof; C J Meijer; A J van den Brule

doi:10.1128/JCM.38.9.3463-3466.2000

Analysis of genetic heterogeneity in Chlamydia trachomatis clinical isolates of serovars D, E, and F by amplified fragment length polymorphism

J Clin Microbiol. 2000 Sep;38(9):3463-6. doi: 10.1128/JCM.38.9.3463-3466.2000.

Authors

S A Morré¹, J M Ossewaarde, P H Savelkoul, J Stoof, C J Meijer, A J van den Brule

Affiliation

¹ Department of Pathology, Section of Molecular Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.

Abstract

Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences were found. Isolates from index patients and partners had identical AFLP patterns and AFLP markers. Characterization of these AFLP markers could give more insight into the differences in virulence and clinical course of C. trachomatis infections.

MeSH terms

Bacterial Outer Membrane Proteins / genetics
Chlamydia Infections / microbiology*
Chlamydia trachomatis / classification*
Chlamydia trachomatis / genetics*
Chlamydia trachomatis / isolation & purification
DNA Fingerprinting / methods*
Female
Female Urogenital Diseases / microbiology
Genetic Variation*
Humans
Male
Male Urogenital Diseases
Polymerase Chain Reaction / methods
Polymorphism, Genetic
Porins*
Serotyping
Urogenital System / microbiology*

Substances

Bacterial Outer Membrane Proteins
Porins
omp1 protein, Chlamydia trachomatis