Abstract
Here we report that the Schizosaccharomyces pombe Rad9 (SpRad9) protein contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. These observations suggest that SpRad9 may represent the first member of the Bcl-2 protein family identified in yeast, though the cell death pathways in S. pombe may differ from those found in mammals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Apoptosis* / drug effects
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Binding Sites
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Cell Cycle Proteins / chemistry*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Division / drug effects
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Cell Line
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Conserved Sequence / genetics
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DNA Damage / drug effects
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DNA Damage / genetics
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Evolution, Molecular
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Gene Deletion
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Gene Expression
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Genes, Suppressor / genetics
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Humans
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Methyl Methanesulfonate / pharmacology
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Mutagens / pharmacology
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Protein Binding
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Protein Structure, Tertiary
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Proto-Oncogene Proteins c-bcl-2 / metabolism*
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Schizosaccharomyces* / cytology
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Schizosaccharomyces* / drug effects
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Schizosaccharomyces* / genetics
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Thiamine / pharmacology
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Two-Hybrid System Techniques
Substances
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Cell Cycle Proteins
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Mutagens
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Proto-Oncogene Proteins c-bcl-2
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rad9 protein
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Methyl Methanesulfonate
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Thiamine