Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis

FEBS Lett. 2000 Sep 15;481(2):147-51. doi: 10.1016/s0014-5793(00)01987-6.

Abstract

SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • CHO Cells
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Cricetinae
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Enzyme Activation
  • Fish Proteins*
  • Gene Expression Regulation
  • Genes, Reporter / genetics
  • Herpes Simplex Virus Protein Vmw65 / chemistry
  • Herpes Simplex Virus Protein Vmw65 / genetics
  • Herpes Simplex Virus Protein Vmw65 / metabolism
  • High Mobility Group Proteins / chemistry
  • High Mobility Group Proteins / genetics
  • High Mobility Group Proteins / metabolism*
  • Leucine Zippers
  • Male
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Organ Specificity
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • SOX Transcription Factors
  • SOXD Transcription Factors
  • Testis / cytology
  • Testis / metabolism*
  • Transcription Factors*
  • Transfection
  • Two-Hybrid System Techniques

Substances

  • DNA-Binding Proteins
  • Fish Proteins
  • Herpes Simplex Virus Protein Vmw65
  • High Mobility Group Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • SOX Transcription Factors
  • SOXD Transcription Factors
  • Solt protein, mouse
  • Sox6 protein, mouse
  • Transcription Factors
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Camk4 protein, mouse

Associated data

  • GENBANK/AB043687