Abstract
SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Binding Sites
-
CHO Cells
-
Calcium-Calmodulin-Dependent Protein Kinase Type 4
-
Calcium-Calmodulin-Dependent Protein Kinases / genetics
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
-
Cricetinae
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Enzyme Activation
-
Fish Proteins*
-
Gene Expression Regulation
-
Genes, Reporter / genetics
-
Herpes Simplex Virus Protein Vmw65 / chemistry
-
Herpes Simplex Virus Protein Vmw65 / genetics
-
Herpes Simplex Virus Protein Vmw65 / metabolism
-
High Mobility Group Proteins / chemistry
-
High Mobility Group Proteins / genetics
-
High Mobility Group Proteins / metabolism*
-
Leucine Zippers
-
Male
-
Mice
-
Molecular Sequence Data
-
Mutation
-
Organ Specificity
-
Promoter Regions, Genetic / genetics
-
Protein Binding
-
RNA, Messenger / analysis
-
RNA, Messenger / genetics
-
Recombinant Fusion Proteins / chemistry
-
Recombinant Fusion Proteins / metabolism
-
SOX Transcription Factors
-
SOXD Transcription Factors
-
Testis / cytology
-
Testis / metabolism*
-
Transcription Factors*
-
Transfection
-
Two-Hybrid System Techniques
Substances
-
DNA-Binding Proteins
-
Fish Proteins
-
Herpes Simplex Virus Protein Vmw65
-
High Mobility Group Proteins
-
RNA, Messenger
-
Recombinant Fusion Proteins
-
SOX Transcription Factors
-
SOXD Transcription Factors
-
Solt protein, mouse
-
Sox6 protein, mouse
-
Transcription Factors
-
Calcium-Calmodulin-Dependent Protein Kinase Type 4
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Camk4 protein, mouse