[Free fatty acids promoting PTP1B expression in rat skeletal muscle and hepatic cells]

Abstract

Objective: To determine whether that free fatty acids (FFAs) impair glucose metabolism is associated with altered insulin signaling system.

Methods: After skeletal muscle and hepatic cells were incubated with palmitate (0.25 mmol/L) or oleate (0.125 mmol/L) for 6, 12 or 24 hours, the protein abundance of SH-PTP2 and PTP1B was assessed by western blot.

Results: SH-PTP2 protein levels showed no significant change in both fatty acids treated muscle and hepatic cells at all time points. The PTP1B was significantly elevated in both liver and muscle cells incubated with FFAs at 6, 12 and 24 hours.

Conclusions: FFAs promote the expression of PTP1B in rat skeletal muscle and liver cells, and the elevated PTP1B may mediate the insulin resistance induced by FFAs.