The human Hsp70B gene at the HSPA7 locus of chromosome 1 is transcribed but non-functional

Biochim Biophys Acta. 2000 Nov 15;1494(1-2):201-5. doi: 10.1016/s0167-4781(00)00203-7.

Abstract

The human heat-inducible Hsp70B and Hsp70B' genes were co-localized to 1q23.1 by in situ hybridization. However, though transcripts from Hsp70B could be detected in heat-shocked cells, DNA sequence analyses of both the gene and cDNA copies of the mRNA indicate the gene is non-functional. Moreover, mouse homologues of Hsp70B/B' were not detected by Southern blot analysis, suggesting Hsp70B/B' arose from either Hsp70-1or Hsp70-2 after the divergence of mice and humans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosomes, Human, Pair 1 / genetics*
  • Evolution, Molecular
  • HSP70 Heat-Shock Proteins / genetics*
  • HSP70 Heat-Shock Proteins / physiology*
  • Heat-Shock Response / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Physical Chromosome Mapping
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic / genetics*
  • Tumor Cells, Cultured

Substances

  • HSP70 Heat-Shock Proteins
  • HSPA7 protein, human
  • RNA, Messenger

Associated data

  • GENBANK/AF093759