Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells

Genes Chromosomes Cancer. 2001 Jan;30(1):57-63. doi: 10.1002/1098-2264(2000)9999:9999<::aid-gcc1054>3.0.co;2-m.

Abstract

In previous studies, we showed that peptide nucleic acid (PNA) probes have significant advantages over conventional synthetic RNA or DNA probes in FISH procedures for detecting telomeric and trinucleotide repeat sequences. Here, we report that directly labeled PNA probes recognizing chromosome-specific repeat sequences are also powerful tools for detecting and enumerating specific chromosomes in interphase and metaphase cells. This is illustrated by multicolor FISH experiments with cells from normal individuals and patients with numerical sex chromosome aberrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines
  • Chromatography, High Pressure Liquid
  • Chromosomes, Human / chemistry*
  • Chromosomes, Human / genetics
  • Female
  • Fibroblasts / chemistry
  • Fluorescent Dyes
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Lymphocytes / chemistry
  • Male
  • Nucleic Acid Probes*
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / genetics
  • Peptide Nucleic Acids / isolation & purification

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Nucleic Acid Probes
  • Peptide Nucleic Acids
  • cyanine dye 3
  • cyanine dye 5