Abstract
We designed a PCR assay specific for the 16S rRNA genes of members of the class Actinobacteria, and created a clone library using the amplification product of total community DNA extracted from anoxic Italian rice field soil. Eighteen out of 27 randomly sequenced clones were affiliated with Actinobacteria, i.e. Frankineae, Corynebacterineae, Micrococcineae, the bacterium candidatus "Microthrix parvicella" and a novel taxonomically undefined cluster.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinobacteria / classification
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Actinobacteria / genetics
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Actinobacteria / isolation & purification*
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Cloning, Molecular
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DNA, Ribosomal / genetics
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DNA, Ribosomal / isolation & purification*
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Evolution, Molecular
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Genes, rRNA
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Italy
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Molecular Sequence Data
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Oryza / microbiology*
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Polymerase Chain Reaction / methods
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RNA, Ribosomal, 16S / genetics*
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Soil Microbiology*
Substances
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/AJ277687
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GENBANK/AJ277688
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GENBANK/AJ277689
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GENBANK/AJ277690
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GENBANK/AJ277691
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GENBANK/AJ277692
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GENBANK/AJ277693
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GENBANK/AJ277694
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GENBANK/AJ277695
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GENBANK/AJ277696
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GENBANK/AJ277697
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GENBANK/AJ277698
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GENBANK/AJ277699
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GENBANK/AJ277700
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GENBANK/AJ277701
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GENBANK/AJ277836
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GENBANK/AJ277837
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GENBANK/AJ277838