Background: There are no established methods for the preparation and standardization of Fusarium solani antigens. This lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests.
Objective: To obtain an appropriate standardized method for the preparation of the different antigen types of F. solani.
Methods: Production of fungal extracts, followed by biochemical and immunological characterization.
Results: The somatic antigens presented the greatest protein content most of these proteins are common to the metabolic and hydrosoluble antigens, particularly those proteins at 35-39 kDa, 29-32 kDa and 15-16 kDa, as detected by electrophoresis and immunoblotting. The hydrosoluble antigens presented the highest protein diversity; these proteins were the most specific, showing minor determinants in common with the other antigens.
Conclusions: It is recommended that a mixture of the different antigen sources be used in order to obtain extracts which would cover the maximum number of diagnostic possibilities.