Synthesis of modified nucleoside 5'-triphosphates for in vitro selection of catalytic nucleic acids

J Matulic-Adamic; A T Daniher; A Karpeisky; P Haeberli; D Sweedler; L Beigelman

doi:10.1081/NCN-100002500

Synthesis of modified nucleoside 5'-triphosphates for in vitro selection of catalytic nucleic acids

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1113-5. doi: 10.1081/NCN-100002500.

Authors

J Matulic-Adamic¹, A T Daniher, A Karpeisky, P Haeberli, D Sweedler, L Beigelman

Affiliation

¹ Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., 2950 Wilderness Place, Boulder, Colorado 80301, USA.

PMID: 11562967
DOI: 10.1081/NCN-100002500

Abstract

2'-Modified pyrimidine nucleoside 5'-triphosphates comprising amino, imidazole and carboxylate functionality attached to the 5-position of the base were synthesized. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates.

MeSH terms

Drug Stability
Pyrimidine Nucleotides / chemical synthesis*
Pyrimidine Nucleotides / chemistry
RNA, Catalytic / chemistry*

Substances

Pyrimidine Nucleotides
RNA, Catalytic