Abstract
The solution-phase, parallel synthesis and evaluation of a library of 132 (+)-1,2,9,9a-tetrahydrocyclopropa[c]benz[e]indol-4-one (CBI) analogues of CC-1065 and the duocarmycins containing dimeric monocyclic, bicyclic, and tricyclic heteroaromatic replacements for the DNA-binding domain are described. This systematic study revealed clear trends in the structural requirements for observation of potent cytotoxic activity and DNA alkylation efficiency, the range of which spans a magnitude of > or =10 000-fold. Combined with related studies, these results highlight that the role of the DNA-binding domain goes beyond simply providing DNA-binding selectivity and affinity (10-100-fold enhancement in properties), consistent with the proposal that it contributes significantly to catalysis of the DNA alkylation reaction accounting for as much as an additional 1000-fold enhancement in properties.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkylating Agents / chemical synthesis
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Alkylating Agents / chemistry
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Alkylating Agents / pharmacology
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Alkylation / drug effects
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Antibiotics, Antineoplastic / chemical synthesis*
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / pharmacology*
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Binding Sites
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Combinatorial Chemistry Techniques
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Cyclopropanes / chemistry
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DNA / metabolism
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DNA, Viral / drug effects
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DNA, Viral / metabolism
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Duocarmycins
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Indoles / chemistry
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Inhibitory Concentration 50
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Leucomycins / chemical synthesis*
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Leucomycins / chemistry
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Leucomycins / pharmacology
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Pyrroles / chemical synthesis
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Pyrroles / chemistry
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Pyrroles / pharmacology
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Pyrrolidinones / chemical synthesis
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Pyrrolidinones / chemistry
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Pyrrolidinones / pharmacology
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Simian virus 40 / genetics
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Structure-Activity Relationship
Substances
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1,2,9,9a-tetrahydrocyclopropa(c)benz(e)indol-4-one
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Alkylating Agents
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Antibiotics, Antineoplastic
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Cyclopropanes
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DNA, Viral
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Duocarmycins
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Indoles
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Leucomycins
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Pyrroles
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Pyrrolidinones
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duocarmycin SA
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CC 1065
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DNA
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duocarmycin A