Abstract
A human cytomegalovirus mutant (TNsubIE2P) was constructed with alanine substitutions of four residues (T27, S144, T233, and S234) previously shown to be phosphorylated in the immediate-early 2 (IE2) protein. This mutant grew as well as the wild type at both low and high multiplicities of infection. The mutant activated the major immediate-early, UL4, and UL44 promoters to similar levels, and with similar kinetics, as wild-type virus. However, the TNsubIE2P mutant virus transactivated an endogenous simian virus 40 early promoter 4 h earlier and to higher levels than the wild-type virus in infected human fibroblasts. The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined.
MeSH terms
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Amino Acid Substitution / genetics
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Cytomegalovirus / genetics*
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Cytomegalovirus / physiology*
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DNA-Binding Proteins / genetics
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Fibroblasts
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Gene Expression Regulation, Viral*
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Genes, Viral / genetics
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Humans
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Immediate-Early Proteins / chemistry
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / metabolism*
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Kinetics
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Membrane Glycoproteins*
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Mutation / genetics*
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Phosphorylation
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Promoter Regions, Genetic / genetics
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RNA, Viral / genetics
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RNA, Viral / metabolism
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SUMO-1 Protein / genetics
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SUMO-1 Protein / metabolism
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Simian virus 40 / genetics
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Trans-Activators*
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Viral Envelope Proteins*
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Viral Proteins / genetics
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Virus Replication
Substances
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DNA-Binding Proteins
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ICP36 protein, Cytomegalovirus
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IE2 protein, Cytomegalovirus
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Immediate-Early Proteins
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Membrane Glycoproteins
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RNA, Viral
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SUMO-1 Protein
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Trans-Activators
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UL115 protein, Human herpesvirus 5
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Viral Envelope Proteins
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Viral Proteins
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glycoprotein H, Cytomegalovirus
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glycoprotein H, Human cytomegalovirus
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glycoprotein O, cytomegalovirus