Abstract
The effect of solR inactivation on the metabolism of Clostridium acetobutylicum was examined using fermentation characterization and metabolic flux analysis. The solR-inactivated strain (SolRH) of this study had a higher rate of glucose utilization and produced higher solvent concentrations (by 25%, 14%, and 81%, respectively, for butanol, acetone, and ethanol) compared to the wild type. Strain SolRH(pTAAD), carrying a plasmid-encoded copy of the bifunctional alcohol/aldehyde dehydrogenase gene (aad) used in butanol production, produced even higher concentrations of solvents (by 21%, 45%, and 62%, respectively, for butanol, acetone, and ethanol) than strain SolRH. Clarithromycin used for strain SolRH maintenance during SolRH(pTAAD) fermentations did not alter product formation; however, tetracycline used for pTAAD maintenance resulted in 90% lower solvent production.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetone / metabolism
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Alcohol Dehydrogenase / genetics
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Aldehyde Dehydrogenase / genetics
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Butanols / metabolism
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Clarithromycin / pharmacology
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Clostridium / genetics
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Clostridium / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Ethanol / metabolism
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Fermentation / drug effects
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Fermentation / genetics*
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Genes, Bacterial
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Genetic Engineering
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Glucose / metabolism
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Industrial Microbiology / methods*
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Models, Biological
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Plasmids / genetics
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Solvents / metabolism*
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Tetracycline / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Butanols
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DNA-Binding Proteins
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Repressor Proteins
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Solvents
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Acetone
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Ethanol
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Alcohol Dehydrogenase
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Aldehyde Dehydrogenase
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Tetracycline
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Clarithromycin
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Glucose