Using the FDD-PCR technique, ten new fiber-specific cDNAs were isolated from developing cotton fiber cells and showed high amino acid identity to previously recorded cDNAs. Five cDNAs encoding bisphosphate nucleotidase, alpha-tubulin, beta-galactosidase, annexin, and reversibly glycosylated polypeptide were identified while the functions of five other cDNAs were undetermined. Dot blot analysis showed that all transcripts of the 10 cDNAs accumulated preferentially in fiber cells and the majority were expressed in the early phase of cotton fiber development, except for F14 which accumulated at a high level during the late phase of developing fiber cells, indicating that all of the genes were involved in the process of fiber development.