In this study, (1) change in bisphenol-A (BPA) leached from polycarbonate (PC) tube to water samples at 37 degrees C, (2) effect of reactive oxygen species (ROS) produced by Fenton reaction on BPA recovery and thiobarbituric acid (TBA) value with or without generally existing environmental substances such as alcohol, lipids and NaCl, were investigated. Amounts of BPA leached from PC tube to water samples containing lipids possessing unsaturated fatty acid with high TBA values were significantly lower than the amount of BPA to water only, and addition of NaCl to lipid containing water further decreased BPA concentration. The result indicates that BPA could be degraded by lipoperoxides formed by auto-oxidation of lipid, and NaCl plays an important role in BPA degradation. In the presence of ROS, BPA recovery was the lowest in water and addition of EtOH increased in both BPA recovery and TBA value, suggesting that EtOH could play a role as scavenger of ROS on the oxidative BPA degradation. Furthermore, the higher the concentration of lipid and/or NaCl, the lower the BPA recovery and TBA value. Physiologically and environmentally important concentrations of NaCl could enhance oxidative degradation of BPA in the presence of ROS.