Objective. To study the effects of the Experimental Closed Suit (ECS) with no active ventilation on human physiological functions and its anti-asphyxic role. Method. Five young healthy male subjects, aged 18-21 years, were tested in the ECS with no active ventilation and no oxygen supply (NAVANOS) when the gas regulatory valve switch was placed on A or B position respectively. Result. (1) Under 1 atm air pressure, each subject could endure at least 15 min while they were aware and resting in the ECS with NAVANOS; (2) There was a balancing period or plateau of the oxygen and the carbon dioxide pressure in the vicinity of the mouth in 5 to 7 min after the sealed helmet was closed, and this period lasted to the end of each test; (3) During the 8-10 min balancing period, the oxygen partial pressure in the vicinity of the mouth ranged from 15.0 to 16.4 kpa, which was slightly hypoxic, and the range of the carbon dioxide partial pressure was 5.21 to 6.49 kPa, which was within the CO2 concentration-time curve of human physiological endurance; (4) No significant difference in human physiological parameters was found when the regulatory valve was placed on A or B position. Conclusion. The anti-asphyxic time of ECS with NAVANOS could at least last 15 min under 1 atm air pressure while the subjects were aware and resting.