Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches

Curr Opin Struct Biol. 2002 Apr;12(2):263-9. doi: 10.1016/s0959-440x(02)00319-6.

Abstract

Intermediate-resolution (7-9A) structures of large macromolecular complexes can be obtained by electron cryomicroscopy. This structural information, combined with bioinformatics data for the individual protein components or domains, can lead to a fold model for the entire complex. Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Cryoelectron Microscopy / methods*
  • Image Processing, Computer-Assisted / methods
  • Macromolecular Substances*
  • Models, Molecular*
  • Protein Folding*
  • Software

Substances

  • Macromolecular Substances