5-propynyluracil.diaminopurine: an efficient base-pair for non-enzymatic transcription of DNA

Chem Commun (Camb). 2002 Aug 7:(15):1568-9. doi: 10.1039/b204535d.

Abstract

The Up.D base-pair (5-propynyl uracil.diaminopurine) is found to be more effective at non-enzymatic transcription than the corresponding natural T.A pair; under non-enzymatic reaction conditions where the natural T.A base-pair fails, a DNA template bearing Up efficiently directs the incorporation of D into a product RNA strand.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 2-Aminopurine / analogs & derivatives*
  • 2-Aminopurine / chemistry*
  • Adenine / chemistry
  • Alkynes / chemistry*
  • Autoradiography
  • Base Pairing
  • DNA Replication
  • Electrophoresis, Polyacrylamide Gel
  • Templates, Genetic
  • Transcription, Genetic*
  • Uracil / analogs & derivatives*
  • Uracil / chemistry*

Substances

  • Alkynes
  • 5-propynyluracil
  • 2-Aminopurine
  • 2,6-diaminopurine
  • Uracil
  • Adenine