Laser capture microdissection of mouse intestine: characterizing mRNA and protein expression, and profiling intermediary metabolism in specified cell populations

Methods Enzymol. 2002:356:167-96. doi: 10.1016/s0076-6879(02)56932-9.

Authors

Thaddeus S Stappenbeck¹, Lora V Hooper, Jill K Manchester, Melissa H Wong, Jeffrey I Gordon

Affiliation

¹ Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

PMID: 12418197
DOI: 10.1016/s0076-6879(02)56932-9

No abstract available

MeSH terms

Animals
Fructose-Bisphosphatase / metabolism
Gene Expression Profiling
Glucose / metabolism
Histocytological Preparation Techniques
Intestinal Mucosa / metabolism*
Intestines / cytology*
Lasers
Mice
Micromanipulation / methods*
Mosaicism
Oligonucleotide Array Sequence Analysis
Proteins / genetics*
Proteins / isolation & purification
RNA, Messenger / genetics*
RNA, Messenger / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction

Substances

Proteins
RNA, Messenger
Fructose-Bisphosphatase
Glucose