Abstract
Objective:
To study the function of THANK in T lymphocyte immune regulation.
Methods:
On the basis of THANK gene cloning and expression, isolated T and B lymphocytes were stimulated by purified THANK protein and its function in immune regulation was investigated by (3)HTdR incorperation and flow cytometric analysis.
Results:
In addition to the costimulation on B cell growth, THANK promoted CD3-mediated T cells proliferation, activated resting peripheral blood T lymphocytes and induced apoptosis and the phenotypic change of activated T lymphocytes.
Conclusion:
THANK is involved in the modulation of T cells phenotype and function.
MeSH terms
-
Antigens, CD / analysis
-
B-Cell Activating Factor
-
B-Lymphocytes / cytology
-
B-Lymphocytes / drug effects
-
B-Lymphocytes / metabolism
-
CD3 Complex / analysis
-
Cell Line
-
Dose-Response Relationship, Drug
-
Flow Cytometry
-
Humans
-
Immunophenotyping
-
Membrane Proteins / genetics
-
Membrane Proteins / pharmacology*
-
Recombinant Proteins / pharmacology
-
T-Lymphocytes / cytology
-
T-Lymphocytes / drug effects*
-
T-Lymphocytes / immunology
-
Thymidine / metabolism
-
Tritium
-
Tumor Necrosis Factor-alpha / genetics
-
Tumor Necrosis Factor-alpha / pharmacology*
Substances
-
Antigens, CD
-
B-Cell Activating Factor
-
CD3 Complex
-
Membrane Proteins
-
Recombinant Proteins
-
TNFSF13B protein, human
-
Tumor Necrosis Factor-alpha
-
Tritium
-
Thymidine