Abstract
In response to T cell activation signals, the half-life of interleukin-2 (IL-2) mRNA is greatly extended. The cis elements mediating IL-2 mRNA stabilization are located in its 5' and 3' untranslated regions (UTR). The 3'UTR also contains AU-rich elements (AREs) that mediate rapid IL-2 mRNA degradation in the cytoplasm of nonstimulated T cells. NF90, a previously described RNA binding protein, binds to a subregion of the 3'UTR that contains several AREs and slows down the degradation of IL-2 mRNA. In nonstimulated cells, NF90 is mostly nuclear, but T cell activation results in its accumulation in the cytoplasm. The nuclear export of NF90 is required for IL-2 mRNA stabilization.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3' Untranslated Regions / genetics
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5' Untranslated Regions / genetics
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Active Transport, Cell Nucleus / physiology*
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Amino Acid Sequence
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Cell Nucleus / metabolism*
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Cytoplasm / immunology
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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HeLa Cells
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Humans
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Interleukin-2 / genetics*
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Jurkat Cells
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Kinetics
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Molecular Sequence Data
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NFATC Transcription Factors
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Nuclear Factor 90 Proteins
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Nuclear Proteins*
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RNA, Messenger / metabolism*
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RNA-Binding Proteins / metabolism
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T-Lymphocytes / immunology*
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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3' Untranslated Regions
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5' Untranslated Regions
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DNA-Binding Proteins
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Interleukin-2
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NFATC Transcription Factors
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Nuclear Factor 90 Proteins
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Nuclear Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Transcription Factors