Abstract
A full length cDNA named HongrES1 was isolated and cloned by screening rat epididymis cDNA library using a mouse EST as a probe and 5'RACE followed. It contained 1590bp nucleotides and its predicted protein had 415 amino acid residues including a serpin (serine protease inhibitor) conserved domain. Tissue distribution pattern showed it was specifically expressed in adult rat epididymis; moreover, in situ hybridyzation indicated this gene was expressed in a limited region of the cauda epididymis near vas deference. Such kind of expression pattern sugested that HongrES1 had potential function in male reproduction.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence / genetics
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Animals
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Base Sequence / genetics
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Cell Differentiation / physiology*
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DNA, Complementary / analysis
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Epididymis / cytology
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Epididymis / metabolism*
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Epithelial Cells / cytology
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Epithelial Cells / metabolism*
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Male
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Molecular Sequence Data
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Reproduction / physiology
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Serpins / genetics
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Serpins / isolation & purification*
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Spermatozoa / cytology
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Spermatozoa / metabolism*
Substances
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DNA, Complementary
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RNA, Messenger
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Serpina16 protein, rat
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Serpins