Abstract
The JAK/STAT signal transduction pathway regulates many developmental processes in Drosophila. However, the functional mechanism of this pathway is poorly understood. In this report, we identify the Drosophila cyclin-dependent kinase 4 (Cdk4), which exhibits embryonic mutant phenotypes identical to those in the Hopscotch/JAK kinase and stat92E/STAT mutations. Specific genetic interactions between Cdk4 and hop mutations suggest that Cdk4 functions downstream of the HOP tyrosine kinase. We further show that Cyclin D-Cdk4 (as well as Cyclin E-Cdk2) binds and regulates STAT92E protein stability. STAT92E regulates gene expression for various biological processes, including the endocycle S phase. These data suggest that Cyclin D-Cdk4 and Cyclin E-Cdk2 play more versatile roles in Drosophila development.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Animals, Genetically Modified
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Body Patterning / genetics*
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CDC2-CDC28 Kinases*
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Cell Differentiation
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Cell Polarity
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Crosses, Genetic
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Cyclin D
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Cyclin E / genetics
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Cyclin E / metabolism*
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases / genetics
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Cyclin-Dependent Kinases / metabolism*
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Cyclins / genetics
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Cyclins / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Drosophila Proteins / metabolism
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Drosophila melanogaster / embryology*
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Drosophila melanogaster / genetics
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Embryo, Nonmammalian / physiology*
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Enzyme Activation
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Eye / cytology
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Eye / embryology
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Female
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Gene Expression Regulation, Developmental
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Glycoproteins / metabolism
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In Situ Hybridization
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Insect Proteins / metabolism
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Janus Kinase 1
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Janus Kinases
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Mutation
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Oogenesis / physiology*
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Ovarian Follicle / cytology
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Ovarian Follicle / physiology*
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Phenotype
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein-Tyrosine Kinases / metabolism*
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Proto-Oncogene Proteins*
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STAT Transcription Factors
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Signal Transduction*
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors*
Substances
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CycD protein, Drosophila
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Cyclin D
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Cyclin E
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Cyclins
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DNA-Binding Proteins
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Drosophila Proteins
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Glycoproteins
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Insect Proteins
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Proto-Oncogene Proteins
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STAT Transcription Factors
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Stat92E protein, Drosophila
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Trans-Activators
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Transcription Factors
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upd1 protein, Drosophila
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Protein-Tyrosine Kinases
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Janus Kinase 1
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Janus Kinases
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hop protein, Drosophila
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Protein Serine-Threonine Kinases
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CDC2-CDC28 Kinases
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Cdk4 protein, Drosophila
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases
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cdk2 protein, Drosophila