Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

Rajesh Babu Sekar; Ammasi Periasamy

doi:10.1083/jcb.200210140

Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

J Cell Biol. 2003 Mar 3;160(5):629-33. doi: 10.1083/jcb.200210140.

Authors

Rajesh Babu Sekar¹, Ammasi Periasamy

Affiliation

¹ W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA.

Abstract

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Eukaryotic Cells / cytology*
Eukaryotic Cells / metabolism
Fluorescence Resonance Energy Transfer / methods*
Fluorescence Resonance Energy Transfer / trends
Fluorescent Dyes*
Green Fluorescent Proteins
Humans
Image Processing, Computer-Assisted / methods
Image Processing, Computer-Assisted / trends
Luminescent Proteins
Microscopy, Fluorescence / methods*
Microscopy, Fluorescence / trends
Proteins / metabolism*

Substances

Fluorescent Dyes
Luminescent Proteins
Proteins
Green Fluorescent Proteins