Crystallization and preliminary X-ray diffraction study of a wheat (Triticum aestivum L.) TAXI-type endoxylanase inhibitor

Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):744-6. doi: 10.1107/s0907444903002890. Epub 2003 Mar 25.

Abstract

A TAXI-type endoxylanase inhibitor from T. aestivum L. wheat flour has been crystallized using the hanging-drop vapour-diffusion method. The needle-like crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 49.92, b = 66.45, c = 106.42 A. From these crystals, a native data set and a gold-derivative data set were collected to 2.25 and 1.75 A resolution, respectively. The heavy-atom derivative of this crystal form was obtained by the soaking method and allowed determination of the initial phases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / isolation & purification
  • Flour / analysis
  • Isoenzymes / chemistry
  • Plant Proteins / chemical synthesis
  • Plant Proteins / pharmacology
  • Substrate Specificity
  • Triticum / chemistry*
  • X-Ray Diffraction

Substances

  • Enzyme Inhibitors
  • Isoenzymes
  • Plant Proteins